HCP antibody

Catalog No. ABIN1607604

Product Details anti-HCP Antibody

Target: HCP (Host Cell Proteins (HCP))

Reactivity: E. coli

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HCP antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: Anti-Host Cell Proteins Antibody recognizes high and low molecular weight protein fractions from E.coli.

Specificity: E.coli HCP Antibody is directed against high molecular weight and low molecular weight E.coli Host Cell Proteins. This whole rabbit antiserum was prepared by delipidation and defibrination followed by the addition of buffer salts and preservative. The anti-sera reactivity is directed against E.coli Host Cell Proteins.

Cross-Reactivity (Details): Cross reactivity with other species is likely but has not been determined. Reactivity against homologues from other sources is not known.

Characteristics: Anti-Host Cell Proteins Antibody recognizes high and low molecular weight protein fractions from E.coli. This antibody has been validated by 2D electrophoresis western blot to assure highest possible coverage of E.coli HCP. Host Cell Proteins are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance. HCP Antibodies are used to detect impurities in biologically-based therapeutics. Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate. Synonyms: Host Cell Protein antibody, HCP antibody, Anti-HCP, Anti-Host Cell Protein, ECP antibody, Anti-ECP

Purification: This whole rabbit antiserum was prepared by delipidation and defibrination followed by the addition of buffer salts and preservative. Reactivity is directed against E.coli Host Cell Proteins.

Sterility: Sterile filtered

Immunogen: Anti-Host Cell Proteins Antibody was prepared combining antibodies that have been separatly developed by repeated immunizations with a mixture of high molecular weight (HMW) and a mixture of low molecular weight (LMW) proteins from E.Coli.
Immunogen Type: Native Protein

Application Details

Application Notes: Western Blot Dilution: 1:500 - 1:1000
ELISA : 1:1000-1:2000

Comment:

Anti-Host Cell Proteins Antibody detects over 300 E.coli proteins and is suitable for western blotting and ELISA.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 77.5 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store Host Cell Proteins Antibody at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for HCP

Target: HCP (Host Cell Proteins (HCP))

Alternative Name: Host Cell Proteins (HCP Products)