KIF11 antibody

Catalog No. ABIN1607602

Product Details anti-KIF11 Antibody

Target: KIF11 (Kinesin Family Member 11 (KIF11))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KIF11 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Cross-Reactivity (Details): Cross reactivity with Eg5 from other sources have not been determined.

Purity: Anti-Eg5 is directed against the human Eg5 protein. The product was prepared from monospecific antiserum by delipidation and defibrination. A BLAST analysis was used to suggest reactivity with human.

Endotoxin Level: Low Endotoxin : No

Immunogen: Eg5 was prepared from whole rabbit serum produced by repeated immunizations with a truncated Eg5 construct expressed in E. coli corresponding to human Eg5 protein.
Immunogen Type: Recombinant Protein

Application Details

Application Notes: Eg5 antibody has been tested for use in ELISA and western blot. For western blots expect a band of approximately 72 kDa in size corresponding to truncated kinesin-1 protein. Specific conditions for reactivity should be optimized by the end user.
ELISA Dilution: 1:10.000
Western Blot Dilution: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 46 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Storage: 4 °C/-20 °C

Storage Comment: Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details for KIF11

Target: KIF11 (Kinesin Family Member 11 (KIF11))

Alternative Name: Eg5 (KIF11 Products)

Synonyms: EG5 antibody, HKSP antibody, KNSL1 antibody, MCLMR antibody, TRIP5 antibody, Eg5 antibody, Kif8 antibody, Kifl1 antibody, Knsl1 antibody, F2H17.19 antibody, F2H17_19 antibody, DDBDRAFT_0189194 antibody, DDBDRAFT_0201556 antibody, DDB_0189194 antibody, DDB_0201556 antibody, XLEg5K1 antibody, XlEg5 antibody, cos2 antibody, costal2 antibody, eg5 antibody, hksp antibody, kif11 antibody, kif11-a antibody, kif11-b antibody, kif11b antibody, knsl1 antibody, trip5 antibody, Cos2 antibody, Costal2 antibody, XLEg5K2 antibody, kif11a antibody, kinesin family member 11 antibody, P-loop containing nucleoside triphosphate hydrolases superfamily protein antibody, kinesin family member 11 S homeolog antibody, kinesin family member 11 L homeolog antibody, KIF11 antibody, Kif11 antibody, AT2G36200 antibody, kif11 antibody, kif11.S antibody, kif11.L antibody

Background: Eg5 (also known as kinesin-5 and KIF11) is a homo-tetramer which cross-links anti-parallel microtubules in the mitotic spindle to maintain spindle bipolarity. Eg5, a member of the Kinesin-5 subclass of kinesins, is a plus-end-directed tetrameric kinesin-family protein that influences the assembly and organization of the mitotic spindle, a self-assembled and dynamic microtubule-based structure that orchestrates chromosome segregation in dividing cells. Eg5 action is essential: when it is depleted from the cytoplasm of meiotically-mature Xenopus laevis eggs, abnormal monopolar spindles form, preventing successful division. Eg5 is expressed in all cells during mitosis and in post-mitotic neurons during development. In developing neurons pharmacological inhibition and siRNA knockdown of Eg5 results in longer axons, more branches, fewer bouts of axon retraction and the inability of growth cones to turn on contact with repulsive substrates. In migratory neurons, inhibition of Eg5 causes neurons to migrate in a random pattern and form shorter leading processes. In adult neurons, Eg5 has a similar effect on inhibiting the rate of short microtubule transport so pharmacological inhibition of adult Eg5 (i.e Monastrol) may be a potential therapeutic tool for the augmentation of adult axon regeneration. Synonyms: Kinesin-5, KIF11

Gene ID: 3799

NCBI Accession: NP_004512

UniProt: P33176