KIF11 antibody

Catalog No. ABIN1607602

Supplier Product No.: 100-401-j04

Product Details anti-KIF11 Antibody

Target: KIF11 (Kinesin Family Member 11 (KIF11))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KIF11 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.: 100-401-j04

Supplier: Rockland

Purpose: Eg5 Antibody

Cross-Reactivity (Details): Anti-Eg5 is directed against the human Eg5 protein.

Characteristics: Synonyms: rabbit anti-Eg5 antibody, Kinesin-like protein KIF11, Kinesin-5, KIF11, Kinesin-related motor protein Eg5, Thyroid receptor-interacting protein 5

Purification: The product was prepared from monospecific antiserum by delipidation and defibrination.

Sterility: Sterile filtered

Immunogen:

Immunogen: Eg5 was prepared from whole rabbit serum produced by repeated immunizations with a truncated Eg5 construct expressed in E. coli corresponding to human Eg5 protein.

Immunogen Type: Recombinant Protein

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: Eg5 antibody has been tested by western blot. For western blots expect a band of approximately 72 kDa in size corresponding to truncated kinesin-1 protein. Specific conditions for reactivity should be optimized by the end user. This antibody is suitable for use in ELISA.

Western Blot Dilution: 1:1000

ELISA Dilution: 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 46 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for KIF11

Target: KIF11 (Kinesin Family Member 11 (KIF11))

Alternative Name: KIF11 (KIF11 Products)

Background: Background: Eg5 (also known as kinesin-5 and KIF11) is a homo-tetramer which cross-links anti-parallel microtubules in the mitotic spindle to maintain spindle bipolarity. Eg5, a member of the Kinesin-5 subclass of kinesins, is a plus-end-directed tetrameric kinesin-family protein that influences the assembly and organization of the mitotic spindle, a self-assembled and dynamic microtubule-based structure that orchestrates chromosome segregation in dividing cells. Eg5 action is essential: when it is depleted from the cytoplasm of meiotically-mature Xenopus laevis eggs, abnormal monopolar spindles form, preventing successful division. Eg5 is expressed in all cells during mitosis and in post-mitotic neurons during development. In developing neurons pharmacological inhibition and siRNA knockdown of Eg5 results in longer axons, more branches, fewer bouts of axon retraction and the inability of growth cones to turn on contact with repulsive substrates. In migratory neurons, inhibition of Eg5 causes neurons to migrate in a random pattern and form shorter leading processes. In adult neurons, Eg5 has a similar effect on inhibiting the rate of short microtubule transport so pharmacological inhibition of adult Eg5 (i.e Monastrol) may be a potential therapeutic tool for the augmentation of adult axon regeneration.

Gene ID: 3799

NCBI Accession: NP_004512

UniProt: P52732