Myosin VI antibody

Catalog No. ABIN1607600

Product Details anti-Myosin VI Antibody

Target: Myosin VI (MYO6)

Reactivity: Human, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Myosin VI antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: Myosin-6 Antibody

Cross-Reactivity (Details): Anti-Myosin-6 is directed against the myosin-6 protein.

Characteristics: Synonyms: rabbit anti-Myosin-6 Antibody, MyosinVI, Unconventional myosin 6

Purification: The product was prepared from monospecific antiserum by delipidation and defibrination.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-Myosin-6 was prepared from whole rabbit serum produced by repeated immunizations with full length myosin-6 construct expressed in SF9 insect cells corresponding to porcine myosyn-6 protein.

Immunogen Type: Recombinant Protein

Application Details

Application Notes:

Application Note: Myosin-6 antibody has been tested by western blot. For western blots expect a band of approximately 150 kDa in size corresponding to full length and ~85 kDa and ~60 kDa for truncated myosin-6 protein. Specific conditions for reactivity should be optimized by the end user. This antibody is suitable for use in ELISA.

Western Blot Dilution: 1:1000

ELISA Dilution: 1:10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 25 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Myosin VI

Target: Myosin VI (MYO6)

Alternative Name: MYO6 (MYO6 Products)

Background: Background: Myosin VI is a myosin superfamily member with unique and intriguing features that allow it to fill a still-expanding number of cell biological roles. This actin-based motor produces force that acts towards the minus end of actin filaments, which is the opposite direction to all other characterized myosins. In mammalian cells, myosin VI is localized to endocytic vesicles, membrane ruffles, the cytosol and the Golgi complex. Its motor function is essential for several physiological functions of the cell, including normal rates of endocytosis, maintenance of Golgi morphology and protein secretion. Myosin VI regulates epithelial cell migration and plays a role in the maintenance of adhesive cellular contacts within epithelial cell layers. It is highly expressed in ovarian cancers and prostate cancers and its expression level, which is upregulated by DNA damage in a p53-dependent manner, correlates with the potential of the tumor to disseminate. More recently, myosin VI has been found involved in EGFR endocytosis through a clathrin dependent mechanism.

Gene ID: 397085

NCBI Accession: NP_999186

UniProt: Q29122

Pathways: Sensory Perception of Sound, Dicarboxylic Acid Transport, Asymmetric Protein Localization