MYH6 antibody

Catalog No. ABIN1607600

Product Details anti-MYH6 Antibody

Target: MYH6 (Myosin Heavy Chain 6, Cardiac Muscle, alpha (MYH6))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MYH6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Cross-Reactivity (Details): Cross reactivity with myosin6 from other sources have not been determined.

Purity: Anti-Myosin-6 is directed against the human myosin-6 protein. The product was prepared from monospecific antiserum by delipidation and defibrination. A BLAST analysis was used to suggest reactivity with human only.

Endotoxin Level: Low Endotoxin : No

Immunogen: Anti-Myosin-6 was prepared from whole rabbit serum produced by repeated immunizations with a truncated myosin-6 construct expressed in SF9 insect cells corresponding to human myosyn-6 protein.
Immunogen Type: Recombinant Protein

Application Details

Application Notes: Myosin-6 antibody has been tested for use in ELISA and western blot. For western blots expect a band of approximately 150 kDa in size corresponding to truncated myosin-6 protein. Specific conditions for reactivity should be optimized by the end user.
ELISA Dilution: 1:10.000
Western Blot Dilution: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 25 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details for MYH6

Target: MYH6 (Myosin Heavy Chain 6, Cardiac Muscle, alpha (MYH6))

Alternative Name: Myosin 6 (MYH6 Products)

Synonyms: Myhca antibody, ASD3 antibody, CMD1EE antibody, CMH14 antibody, MYHC antibody, MYHCA antibody, SSS3 antibody, alpha-MHC antibody, A830009F23Rik antibody, AA517445 antibody, Myhc-a antibody, amhc antibody, MYH6 antibody, P-MHC antibody, VMHC1 antibody, myosin antibody, myosin heavy chain 6 antibody, myosin, heavy polypeptide 6, cardiac muscle, alpha antibody, myosin, heavy chain 6, cardiac muscle, alpha antibody, myosin-6 antibody, myosin, heavy chain 15 antibody, Myh6 antibody, MYH6 antibody, LOC101120580 antibody, myh6 antibody, MYH15 antibody

Background: Myosin VI is a myosin superfamily member with unique and intriguing features that allow it to fill a still-expanding number of cell biological roles. This actin-based motor produces force that acts towards the minus end of actin filaments, which is the opposite direction to all other characterized myosins. In mammalian cells, myosin VI is localized to endocytic vesicles, membrane ruffles, the cytosol and the Golgi complex. Its motor function is essential for several physiological functions of the cell, including normal rates of endocytosis, maintenance of Golgi morphology and protein secretion. Myosin VI regulates epithelial cell migration and plays a role in the maintenance of adhesive cellular contacts within epithelial cell layers. It is highly expressed in ovarian cancers and prostate cancers and its expression level, which is upregulated by DNA damage in a p53-dependent manner, correlates with the potential of the tumor to disseminate. More recently, myosin VI has been found involved in EGFR endocytosis through a clathrin dependent mechanism. Synonyms: MyosinVI, Unconventional myosin 6

Gene ID: 3799

NCBI Accession: NP_004512

UniProt: P33176