ARFGAP3 antibody

Catalog No. ABIN1607598

Supplier Product No.: 100-401-h46

Product Details anti-ARFGAP3 Antibody

Target: ARFGAP3 (ADP-Ribosylation Factor GTPase Activating Protein 3 (ARFGAP3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARFGAP3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Supplier Product No.: 100-401-h46

Supplier: Rockland

Purpose: ARFGAP3 Antibody

Cross-Reactivity (Details): Further purification was used to remove the GST tag.

Characteristics: Synonyms: rabbit anti-ArfGAP3 Antibody, ARFGAP1, ADP-ribosylation factor GTPase-activating protein 3, ARF GAP3, Arf-GAP, ArfGAP

Purification: Anti-ArfGAP3 antibody was prepared from monospecific antiserum by delipidation and defibrination.

Sterility: Sterile filtered

Immunogen:

Immunogen: This whole rabbit serum was prepared by repeated immunizations with a truncated recombinant sequence of ArfGAP3 fused to GST.

Immunogen Type: Recombinant Protein

Application Details

Application Notes:

Application Note: ArfGAP3 has been tested for use in Immunofluorescence and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 57 kDa in size by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:1,000

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: 1:5,000

IF Microscopy Dilution: 1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for ARFGAP3

Target: ARFGAP3 (ADP-Ribosylation Factor GTPase Activating Protein 3 (ARFGAP3))

Alternative Name: ARFGAP3 (ARFGAP3 Products)

Background: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. The ArfGAPs are a family of proteins encoded by 31 genes in humans. The function of the ArfGAPs is to regulate the small G protein Arf, a regulator of membrane traffic and actin cytoskeleton. The ArfGAPs have been found to be key regulators of cellular behaviors that involve coordinated actin and membrane remodeling, including protein secretion and migration. Several ArfGAPs are associated with cancer cell invasion and metastasis, and the Arf pathway has been found to be affected in a number of genetic diseases. Although the importance of the ArfGAPs and the Arf pathway for cellular physiology is well recognized, the molecular basis for the function of these proteins has not been established. Reagents for the studies, especially antibodies specific for particular members of the ArfGAP family that can be used for immunoblotting and immunofluorescence, are not available. ArfGAP3 is one of three ArfGAPs thought to be specifically associated with the Golgi apparatus, however, localization has only been done for ectopically expressed recombinant protein and cellular function is still not established. Indeed, there is a growing controversy about the function of ArfGAP3 together with ArfGAP1 and ArfGAP2. Antibodies suitable for immunoprecipitation, immunoblotting and immunofluorescence would be of great value in addressing the controversy and for discovering the potential role of ArfGAP3 in secretion of proteins, such as growth factors, and trafficking of other proteins to the cell surface, such as growth factor receptors and cell adhesion molecules.

Gene ID: 26286

NCBI Accession: NP_001135765

UniProt: Q9NP61