ARFGAP3 antibody

Catalog No. ABIN1607598

Product Details anti-ARFGAP3 Antibody

Target: ARFGAP3 (ADP-Ribosylation Factor GTPase Activating Protein 3 (ARFGAP3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARFGAP3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Cross-Reactivity (Details): Cross reactivity with human, monkey, and orangutan for ArfGAP3. Cross reactivity with AfGAP3 from other sources have not been determined.

Purity: Anti-ArfGAP3 atibody was prepared from monospecific antiserum by delipidation and defibrination. Further purification was used to remove the GST tag. The antibody detects ArfGAP3 in cell lysates.

Endotoxin Level: Low Endotoxin : No

Immunogen: This whole rabbit serum was prepared by repeated immunizations with a truncated recombinant sequence of ArfGAP3 fused to GST.
Immunogen Type: Recombinant Protein

Application Details

Application Notes: ArfGAP3 has been tested for use in ELISA, Immunofluorescence, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 57 kDa in size by western blotting in the appropriate cell lysate or extract.
ELISA Dilution: 1:5.000
IF Microscopy Dilution: 1:100
Western Blot Dilution: 1:1.000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details for ARFGAP3

Target: ARFGAP3 (ADP-Ribosylation Factor GTPase Activating Protein 3 (ARFGAP3))

Alternative Name: ArfGAP3 (ARFGAP3 Products)

Synonyms: ARFGAP1 antibody, 0610009H19Rik antibody, 1810004P07Rik antibody, 1810035F16Rik antibody, 9130416J18Rik antibody, Arfgap1 antibody, arfgap1 antibody, xgap antibody, zgc:85765 antibody, arfgap3 antibody, ADP ribosylation factor GTPase activating protein 3 antibody, ADP-ribosylation factor GTPase activating protein 3 antibody, ADP ribosylation factor GTPase activating protein 3 S homeolog antibody, ARFGAP3 antibody, Arfgap3 antibody, arfgap3 antibody, arfgap3.S antibody

Background: The ArfGAPs are a family of proteins encoded by 31 genes in humans. The function of the ArfGAPs is to regulate the small G protein Arf, a regulator of membrane traffic and actin cytoskeleton. The ArfGAPs have been found to be key regulators of cellular behaviors that involve coordinated actin and membrane remodeling, including protein secretion and migration. Several ArfGAPs are associated with cancer cell invasion and metastasis, and the Arf pathway has been found to be affected in a number of genetic diseases. Although the importance of the ArfGAPs and the Arf pathway for cellular physiology is well recognized, the molecular basis for the function of these proteins has not been established. Reagents for the studies, especially antibodies specific for particular members of the ArfGAP family that can be used for immunoblotting and immunofluorescence, are not available. ArfGAP3 is one of three ArfGAPs thought to be specifically associated with the Golgi apparatus, however, localization has only been done for ectopically expressed recombinant protein and cellular function is still not established. Indeed, there is a growing controversy about the function of ArfGAP3 together with ArfGAP1 and ArfGAP2. Antibodies suitable for immunoprecipitation, immunoblotting and immunofluorescence would be of great value in addressing the controversy and for discovering the potential role of ArfGAP3 in secretion of proteins, such as growth factors, and trafficking of other proteins to the cell surface, such as growth factor receptors and cell adhesion molecules. Synonyms: ARFGAP1, ADP-ribosylation factor GTPase-activating protein 3, ARF GAP3, Arf-GAP

Gene ID: 26286

NCBI Accession: NP_001135765

UniProt: Q9NP61