TNNI3 antibody (AA 117-127)

Catalog No. ABIN1590057

Product Details anti-TNNI3 Antibody

Target: TNNI3 (Cardiac Troponin I (TNNI3))

Binding Specificity: AA 117-127

Reactivity: Human, Mouse, Rat, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This TNNI3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Purpose: TNNI3 (aa117-127)

Sequence: KVTKNITEIA D

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 25+26 kDa doublet band observed in Human Heart, approx 24+27 kDa in Mouse Heart, approx 25 kDa in Rat Heart and approx 27 kDa in Pig Heart lysates (calculated MW of 24 kDa according to Human NP_000354.4, and Pig NP_001092069.1 and 24.3 kDa

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and HeLa cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for TNNI3

Target: TNNI3 (Cardiac Troponin I (TNNI3))

Alternative Name: TNNI3 (TNNI3 Products)

Background: TNNI3, troponin I type 3 (cardiac), CMD1FF, CMD2A, CMH7, RCM1, TNNC1, cTnI, troponin I, cardiac muscle

Gene ID: 7137, 21954, 29248

NCBI Accession: NP_000354