BCAR1 antibody (Internal Region)

Catalog No. ABIN1590037

Product Details anti-BCAR1 Antibody

Target: BCAR1 (Breast Cancer Anti-Estrogen Resistance 1 (BCAR1))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This BCAR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purpose: BCAR1

Sequence: KQTSTFSKQT PHH

Specificity: This antibody is expected to recognize reported isoforms1, 2, 3, 5, 6 and 7.

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Uterus shows strong staining at the glandular side of the endometrial epithelium. Recommended concentration, 5-10 μg/mL.

Western Blot: Approx 100 kDa band observed in lysates of Human breast cancer and of cell line MCF7 (calculated MW of 97.9 kDa according to NP_001164185.1). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for BCAR1

Target: BCAR1 (Breast Cancer Anti-Estrogen Resistance 1 (BCAR1))

Alternative Name: BCAR1 (BCAR1 Products)

Synonyms: CAS antibody, CAS1 antibody, CASS1 antibody, CRKAS antibody, P130Cas antibody, AI385681 antibody, Cas antibody, Crkas antibody, P130CAS antibody, MGC68466 antibody, cas antibody, p130cas antibody, BCAR1 antibody, fc17f11 antibody, wu:fc17f11 antibody, zgc:175192 antibody, BCAR1, Cas family scaffolding protein antibody, breast cancer anti-estrogen resistance 1 antibody, breast cancer anti-estrogen resistance 1 L homeolog antibody, breast cancer anti-estrogen resistance protein 1 antibody, BCAR1 antibody, Bcar1 antibody, bcar1.L antibody, bcar1 antibody, LOC100435586 antibody

Background: BCAR1, breast cancer anti-estrogen resistance 1, CAS, CAS1, CASS1, CRKAS, P130Cas, Cas scaffolding protein family member 1, Crk-associated substrate p130Cas, breast cancer anti-estrogen resistance protein 1

Gene ID: 9564, 12927, 25414

NCBI Accession: NP_001164185, NP_001164186, NP_001164187, NP_001164188, NP_001164189, NP_055382, NP_001164190, NP_001164191, NP_001164192

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events, Platelet-derived growth Factor Receptor Signaling