ITK antibody (Internal Region)

Catalog No. ABIN1589997

Product Details anti-ITK Antibody

Target: ITK (IL2-Inducible T-Cell Kinase (ITK))

Binding Specificity: Internal Region

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This ITK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: ITK

Sequence: KERPEDRPAF SR

Cross-Reactivity: Cow, Human, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 70 kDa band observed in Rat Colon, Lung and Spleen lysates (calculated MW of 72.4 kDa according to Rat NP_001102295.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and membranes of Jurkat cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concen

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ITK

Target: ITK (IL2-Inducible T-Cell Kinase (ITK))

Alternative Name: ITK (ITK Products)

Synonyms: Emt antibody, Tcsk antibody, Tsk antibody, EMT antibody, LPFS1 antibody, LYK antibody, PSCTK2 antibody, IL2 inducible T cell kinase antibody, IL2 inducible T-cell kinase antibody, IL2-inducible T-cell kinase antibody, Itk antibody, ITK antibody

Background: ITK, IL2-inducible T-cell kinase, EMT, LYK, MGC126257, MGC126258, PSCTK2, homolog of mouse T-cell itk/tsk, tyrosine-protein kinase ITK/TSK, tyrosine-protein kinase LYK

Gene ID: 3702

NCBI Accession: NP_005537

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway