MESP1 antibody

Catalog No. ABIN1589957

Product Details anti-MESP1 Antibody

Target: MESP1 (Mesoderm Posterior 1 (MESP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MESP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: MesP1 antibody

Specificity: Recombinant human MesP1

Characteristics: Chromosomal location: 15q26.1

Purification: Protein A purified

Immunogen: Recombinant human MesP1

Isotype: IgG

Application Details

Application Notes: Western Blot: Use 1-5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS

Handling Advice: Centrifuge vial prior to opening.

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

Expiry Date: 24 months

Target Details for MESP1

Target: MESP1 (Mesoderm Posterior 1 (MESP1))

Alternative Name: MesP1 (MESP1 Products)

Synonyms: bHLHc5 antibody, mesp antibody, mesp1 antibody, mespa antibody, CMESO-1 antibody, CMESO2 antibody, MESP1 antibody, mesoderm posterior 1 antibody, mesoderm posterior bHLH transcription factor 1 antibody, mesoderm posterior 1 homolog (mouse) antibody, mesoderm posterior homolog B antibody, mesoderm posterior homolog B L homeolog antibody, Mesp1 antibody, MESP1 antibody, mesp1 antibody, mespb antibody, mespb.L antibody

Background: MESP1, bHLHc5,ES-cell-based cardiovascular repair requires an in-depth understanding of the molecular mechanisms underlying the differentiation of cardiovascular ES cells. A candidate cardiovascular-fate inducer is the bHLH transcription factor MesP1. As one of the earliest markers, it is expressed specifically in almost all cardiovascular precursors and is required for cardiac morphogenesis. It was shown that MesP1 is a key factor sufficient to induce the formation of ectopic heart tissue in vertebrates and increase cardiovasculogenesis by ES cells. Electrophysiological analysis showed all subtypes of cardiac ES-cell differentiation. MesP1 overexpression and knockdown experiments revealed a prominent function of MesP1 in a gene regulatory cascade, causing Dkk-1-mediated blockade of canonical Wnt-signalling. Independent evidence from ChIP and in vitro DNA-binding studies, expression analysis in wild-type and MesP knockout mice, and reporter assays confirm that Dkk-1 is a direct target of MesP1.

Gene ID: 55897

NCBI Accession: NM_018670, NP_061140

UniProt: Q9BRJ9

Pathways: Regulation of Muscle Cell Differentiation