VEGFC antibody

Catalog No. ABIN1589951

Product Details anti-VEGFC Antibody

Target: VEGFC (Vascular Endothelial Growth Factor C (VEGFC))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This VEGFC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: VEGF-C antibody

Specificity: Recombinant human VEGF-C

Cross-Reactivity: Rat

Cross-Reactivity (Details): This antibody detects human and rat VEGF-C in Western blots. No cross-reactivity is shown with VEGF-A.

Characteristics: Chromosomal location: 11q13
This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with recombinant human VEGF-C recombinant protein).

Purification: The IgG2a fraction of culture supernatant was purified by Protein G affinity chromatography.

Immunogen: Recombinant human VEGF-C

Clone: 9E7

Isotype: IgG2a

Application Details

Application Notes: Western Blot: Use at 1-5 μg/mL, IHC: Use at 2-10 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS

Handling Advice: Centrifuge vial prior to opening.

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

Expiry Date: 24 months

Target Details for VEGFC

Target: VEGFC (Vascular Endothelial Growth Factor C (VEGFC))

Alternative Name: VEGF-C (VEGFC Products)

Background: Vascular endothelial growth factor C, VEGFC, VRP, Flt4-L,VEGF-C, also known as Vascular Endothelial Growth Factor Related Protein (VRP), is a recently discovered VEGF growth factor family member that is most closely related to VEGF-D. The human VEGF-C cDNA encodes a pre-pro-protein of 416 amino acids residues. It is almost identical to the mouse VEGF-C protein. Similar to VEGF-D, VEGF-C has a VEGF homology domain spanning the middle third of the precursor molecule and long N- and C-terminal extensions. In adults, VEGF-C is highly expressed in heart, placenta, ovary and small intestine. Recombinant human VEGF-C, lacking the N- and C-terminal extensions and containing only the middle VEGF homology domain, forms primarily non-covalently linked dimers. This protein is a ligand for both VEGFR-2/KDR and VEGFR-3/FLT-4. Since VEGFR-3 is strongly expressed in lymphatic endothelial cells, it has been postulated that VEGF-C is involved in the regulation of the growth and/or differentiation of lymphatic endothelium. Although recombinant human VEGF-C is also a mitogen for vascular endothelial cells, it is much less potent than VEGF-A. The recombinant human VEGF-C contains 115 amino acids residues and was fused to a His-tag (6x His) at the C-terminal end. As a result of glycosylation VEGF-C migrates as an 18-24 kDa protein in SDS-PAGE under reducing conditions.

Gene ID: 7424

NCBI Accession: NM_005429, NP_005420

UniProt: P49767

Pathways: RTK Signaling, Signaling Events mediated by VEGFR1 and VEGFR2