ESAM antibody

Catalog No. ABIN1589920

Product Details anti-ESAM Antibody

Target: ESAM (Endothelial Cell Adhesion Molecule (ESAM))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ESAM antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)

Purpose: ESAM antibody

Specificity: Recombinant human ESAM

Characteristics: Chromosomal location: 11q24.2
Produced from sera of rabbits immunized with highly pure recombinant human soluble ESAM (Endothelial cell-selective adhesion molecule, Q30-A247, SwissProt: #Q96AP7) as the immunizing antigen.

Purification: Anti-human ESAM was purified by affinity chromatography with immobilized Protein A.

Immunogen: Recombinant human ESAM (ABIN1589731)

Isotype: IgG

Application Details

Application Notes: Western Blot: Use 2-5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS

Handling Advice: Centrifuge vial prior to opening. Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

Expiry Date: 24 months

Target Details for ESAM

Target: ESAM (Endothelial Cell Adhesion Molecule (ESAM))

Alternative Name: ESAM (ESAM Products)

Synonyms: ESAM antibody, sc:d0254 antibody, W117m antibody, 2310008D05Rik antibody, Esam1 antibody, endothelial cell adhesion molecule antibody, endothelial cell adhesion molecule a antibody, endothelial cell-specific adhesion molecule antibody, ESAM antibody, esama antibody, Esam antibody

Background: ESAM, W117m,Endothelial cell selective adhesion molecule (ESAM) is a 55 kDa type I transmembrane glycoprotein that belongs to the JAM family of immunoglobulin superfamily molecules. Human ESAM is synthesized as a 390 amino acid (aa) protein composed of a 29 aa signal peptide, a 216 aa extracellular region, a putative 26 aa transmembrane segment, and a 119 aa cytoplasmic domain. The extracellular region contains one V-type and one C2-type Ig domain and is involved in hemophilic adhesion. In the cytoplasmic domain, there is a docking site for the multifunctional adaptor protein MAGI1. The extracellular region of human ESAM shows 90 % , 74 %, 69 % and 67 % aa identity with monkey, canine, mouse and rat extracellular ESAM, respectively. ESAM is expressed on endothelial cells, activated platelets and megakaryocytes, and can be found associated with cell to cell junctions. Whether ESAM is restricted to a particular junctional type is not clear. ESAM deficient mice have no defect in vascularization but do have reduced angiogenic potential. This may be due to a decreased migratory response to FGF2.

Gene ID: 90952

NCBI Accession: NM_138961, NP_620411

UniProt: Q96AP7