PROX1 antibody

Catalog No. ABIN1589859

Product Details anti-PROX1 Antibody

Target: PROX1 (Prospero Homeobox 1 (PROX1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PROX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Purpose: Prox-1 antibody

Specificity: Recombinant human Prox-1

Characteristics: Chromosomal location: 1q32.2-q32.3

Purification: Protein A purified

Immunogen: Recombinant human Prox-1 (ABIN1589681)

Isotype: IgG

Application Details

Application Notes: Western Blot: Use 1-5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: 0.5X PBS, pH 7.2

Handling Advice: Centrifuge vial prior to opening. Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

Expiry Date: 24 months

References for anti-PROX1 antibody (ABIN1589859)

Lee, Koh: "Coordinated lymphangiogenesis is critical in lymph node development and maturation." in: Developmental dynamics : an official publication of the American Association of Anatomists, (2016) (PubMed).

Quagliata, Klusmeier, Cremers, Pytowski, Harvey, Pettis, Thiele, Sleeman: "Inhibition of VEGFR-3 activation in tumor-draining lymph nodes suppresses the outgrowth of lymph node metastases in the MT-450 syngeneic rat breast cancer model." in: Clinical & experimental metastasis, Vol. 31, Issue 3, pp. 351-65, (2014) (PubMed).

Kaltezioti, Antoniou, Stergiopoulos, Rozani, Rohrer, Politis: "Prox1 regulates Olig2 expression to modulate binary fate decisions in spinal cord neurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 34, Issue 47, pp. 15816-31, (2014) (PubMed).

Sleeckx, Van Brantegem, Van den Eynden, Fransen, Casteleyn, Van Cruchten, Veldhuis Kroeze, Van Ginneken: "Angiogenesis in canine mammary tumours: a morphometric and prognostic study." in: Journal of comparative pathology, Vol. 150, Issue 2-3, pp. 175-83, (2014) (PubMed).

Trindade, Torrelo, Kutzner, Requena, Tellechea, Colmenero: "An immunohistochemical study of angiokeratomas of children." in: The American Journal of dermatopathology, Vol. 36, Issue 10, pp. 796-9, (2014) (PubMed).

Benevenuto de Andrade, Ramírez-Amador, Anaya-Saavedra, Martínez-Mata, Fonseca, Graner, Paes de Almeida: "Expression of PROX-1 in oral Kaposi's sarcoma spindle cells." in: Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology, Vol. 43, Issue 2, pp. 132-6, (2014) (PubMed).

Dartsch, Schulte, Hägerling, Kiefer, Vestweber: "Fusing VE-cadherin to ?-catenin impairs fetal liver hematopoiesis and lymph but not blood vessel formation." in: Molecular and cellular biology, Vol. 34, Issue 9, pp. 1634-48, (2014) (PubMed).

Burger, Stuurman, Kok, Konijn, Schooneman, Niederreither, Coles, Agace, Christoffels, Mebius, van de Pavert, Bekker: "Involvement of neurons and retinoic acid in lymphatic development: new insights in increased nuchal translucency." in: Prenatal diagnosis, Vol. 34, Issue 13, pp. 1312-9, (2014) (PubMed).

Buttler, Badar, Seiffart, Laggies, Gross, Wilting, Weich: "De novo hem- and lymphangiogenesis by endothelial progenitor and mesenchymal stem cells in immunocompetent mice." in: Cellular and molecular life sciences : CMLS, Vol. 71, Issue 8, pp. 1513-27, (2014) (PubMed).

Target Details for PROX1

Target: PROX1 (Prospero Homeobox 1 (PROX1))

Alternative Name: Prox-1 (PROX1 Products)

Synonyms: xprox1 antibody, prospero antibody, PROX1 antibody, LOC100232600 antibody, A230003G05Rik antibody, prox1 antibody, zgc:111888 antibody, prospero homeobox 1 antibody, prospero homeobox 1 L homeolog antibody, prospero homeobox 1a antibody, PROX1 antibody, prox1.L antibody, prox1 antibody, Prox1 antibody, prox1a antibody

Background: PROX1, W117m,Prox-1 is a homeobox gene and acts as a master switch for lymphatic endothelial phenotype. Expression of Prox-1 in blood endothelial cells induces expression of other lymphatic marker genes. Together with Podoplanin, Prox-1 can be used to reliably distinguish lymphatic vessels from blood vessels. Prox-1 is expressed in CNS, eye, pancreas, liver and heart, and it is one of the most specific and reliable markers for lymphatic endothelial cells. Produced from sera of rabbits immunized with the recombinant highly conserved C-terminal part of the homeobox transcription factor Prox-1.

Gene ID: 5629

NCBI Accession: NM_002763, NP_002754

UniProt: Q92786

Pathways: Stem Cell Maintenance, Regulation of Muscle Cell Differentiation