Podoplanin antibody

Catalog No. ABIN1589802

Product Details anti-Podoplanin Antibody

Target: Podoplanin (PDPN)

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Podoplanin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: Podoplanin antibody

Specificity: Membrane protein fraction of isolated rat glomeruli

Characteristics: Chromosomal location: 5q36
Monoclonal antibodies were produced with the help of BALB/c mice using a membrane protein fraction of isolated rat glomeruli as the immunizing antigen.

Purification: The mouse IgG antibody (#LF3(B7)D5B3) from hybridomas was purified from cell culture supernatant by Protein G chromatography.

Immunogen: Membrane protein fraction of isolated rat glomeruli

Clone: LF3(B7D5B3)

Isotype: IgG

Application Details

Application Notes: Western Blot: use at 1-5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS

Handling Advice: Centrifuge vial prior to opening.

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

Expiry Date: 24 months

References for anti-Podoplanin antibody (ABIN1589802)

Weinkopff, Mackenzie, Eversole, Lammie: "Filarial excretory-secretory products induce human monocytes to produce lymphangiogenic mediators." in: PLoS neglected tropical diseases, Vol. 8, Issue 7, pp. e2893, (2014) (PubMed).

Target Details for Podoplanin

Target: Podoplanin (PDPN)

Alternative Name: Podoplanin (PDPN Products)

Synonyms: PDPN antibody, AGGRUS antibody, GP36 antibody, GP40 antibody, Gp38 antibody, HT1A-1 antibody, OTS8 antibody, PA2.26 antibody, T1A antibody, T1A-2 antibody, OTS-8 antibody, RANDAM-2 antibody, T1-alpha antibody, T1a antibody, T1alpha antibody, E11 antibody, RTI40 antibody, podoplanin antibody, PDPN antibody, Pdpn antibody

Background: Pdpn, E11, Gp38, OTS-8, RTI40, T1-alpha,Podoplanin is a well-recognized lymphatic endothelium marker, which can be used to reliably distinguish lymphatic vessels from blood vessels. It was originally discovered in kidney podocytes. This mouse monoclonal antibody (IgG) against rat podoplanin works well for immunohistochemistry with formalin-fixed paraffin embedded sections. This antibody also can be used in FACS sorting.

Gene ID: 54320

NCBI Accession: NM_019358, NP_062231

UniProt: Q64294

Pathways: Dicarboxylic Acid Transport