VEGF-Apan (Peptide) (N-Term) antibody
Reactivity: Human
IP, WB
Host: Rabbit
Polyclonal
unconjugated
-
- Target
- VEGF-Apan (Peptide)
- Binding Specificity
- N-Term
- Reactivity
- Human
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- Un-conjugated
- Application
- Immunoprecipitation (IP), Western Blotting (WB)
- Purpose
- VEGF-Apan (Peptide) antibody
- Specificity
- N-terminal peptide (20aa) of native human VEGF165
- Characteristics
-
Chromosomal location: 6p12
Produced from sera of rabbits immunized with a highly purified N-terminal peptide of human VEGF-A. - Purification
- Anti-VEGF-A was purified by affinity chromatography with immobilized Protein A.
- Immunogen
- N-terminal peptide (20aa) of native human VEGF165
- Isotype
- IgG
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-
- Application Notes
- IP: Use 1-5 μg/mL
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.
- Buffer
- PBS
- Handling Advice
- Centrifuge vial prior to opening.
- Storage
- 4 °C,-20 °C
- Storage Comment
- The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.
- Expiry Date
- 24 months
-
- Target
- VEGF-Apan (Peptide)
- Background
- VEGFA, VPF, VEGF, MVCD1,Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF) or vasculotropin, is a homodimeric 34 - 42 kDa, heparin-binding glycoprotein with potent angiogenic, mitogenic and vascular permeability-enhancing activities specific for endothelial cells. Different isoforms can be generated by differential splicing (e.g. VEGF165). All eight cysteine residues involved in intra- and inter-chain disulfide bonds are conserved among these growth factors. A cDNA encoding a protein having a 53 % amino acid sequence homology in the PDGF-like region of VEGF has been isolated from a human placental cDNA library. This protein, named placenta growth factor (PlGF), is now recognized to be a member of the VEGF family of growth factors. Two receptor tyrosine kinases have been described as putative VEGF receptors. Flt-1 (fms-like tyrosine kinase), and KDR (kinase-insert-domain-containing receptor) proteins have been shown to bind VEGF-A with high affinity. In vitro, VEGF is a potent endothelial cell mitogen. The antibody is against the N-terminus and will recognize all VEGF-A isoforms.
- Gene ID
- 7422
- NCBI Accession
- NM_001171626, NP_001165097
- UniProt
- P15692
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