Podoplanin antibody

Catalog No. ABIN1589723

Product Details anti-Podoplanin Antibody

Target: Podoplanin (PDPN)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Podoplanin antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Purpose: Podoplanin antibody

Specificity: Membrane protein fraction of isolated human glomeruli

Characteristics: Chromosomal location: 1p36.21
The monoclonal antibody was produced with the help of BALB/c mice using a membrane protein fraction of isolated human glomeruli as the immunizing antigen.

Purification: The mouse IgG1, kappa antibody (#4D5aE5E6) from hybridomas was purified from cell culture supernatant by Protein G chromatography.

Immunogen: Membrane protein fraction of isolated human glomeruli

Clone: 4D5aE5E6

Isotype: IgG1, kappa

Application Details

Application Notes: Immunohistochemistry: Cryo sections (acetone fixed), Paraffin sections (after autoclave or microwave treatment in 0.01 M citrate buffer, pH 6.0). Recommended working concentration is 0.5 μg/mL. WB: Use at 1-5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS

Handling Advice: Centrifuge vial prior to opening.

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

Expiry Date: 24 months

References for anti-Podoplanin antibody (ABIN1589723)

Steiniger, Wilhelmi, Seiler, Lampp, Stachniss: "Heterogeneity of stromal cells in the human splenic white pulp. Fibroblastic reticulum cells, follicular dendritic cells and a third superficial stromal cell type." in: Immunology, Vol. 143, Issue 3, pp. 462-77, (2014) (PubMed).

Target Details for Podoplanin

Target: Podoplanin (PDPN)

Alternative Name: Podoplanin (PDPN Products)

Background: PDPN, T1A, GP36, GP40, Gp38, OTS8, T1A-2, AGGRUS, HT1A-1, PA2.26,Podoplanin, also known as glycoprotein 36 (gp36), PA2.26 antigen, T1alpha (T1A), and aggrus, is a 36 kDa type I transmembrane sialoglycoprotein and member of the Podoplanin family. Podoplanin has three potential splice variants, the longest of which is represented by a 238 amino acid (aa) precursor (NP_006465). It contains an undefined signal sequence, a 22 aa transmembrane segment (aa 207-228) and a short cytoplasmic tail (aa 229-238). The ECD contains abundant Ser/Thr residues that could serve as potential O-linked glycosolation sites. The cytoplasmic tail contains putative sites for protein kinase C phosphorylation. There are two potential alternate start sites at Met 77 (Swiss Prot #: Q86YL7) and Met 119 (EAW51692) that generate short forms. The 162 aa short form Podoplanin precursor shares 47 % aa identity with mouse Podoplanin. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells, and numerous tumors, including colorectal tumors, squamous cell carcinomas, testicular seminoma, and brain tumors. One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver. Podoplanin is the ligand for C-type lectin-like receptor 2 (CLEC2). Their association is dependent on sialic acid on O-glycans of Podoplanin. Through its association with CLEC2, Podoplanin-induces platelet aggregation and tumor metastasis. Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth.

Gene ID: 10630

NCBI Accession: NM_001006624, NP_001006625

UniProt: Q86YL7

Pathways: Dicarboxylic Acid Transport