DDX60 antibody
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- Target See all DDX60 Antibodies
- DDX60 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 60 (DDX60))
- Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This DDX60 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunofluorescence (IF)
- Specificity
- At least three isoforms of DDX60 are known to exist, this antibody will detect all three isoforms.
- Purification
- Affinity chromatography purified via peptide column
- Immunogen
- DDX60 antibody was raised against a 17 amino acid synthetic peptide near the center of human DDX60.
- Isotype
- IgG
- Top Product
- Discover our top product DDX60 Primary Antibody
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- Application Notes
- DDX60 antibody can be used for detection of DDX60 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
- Comment
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(IN) DEAD box protein 60
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS containing 0.02 % Sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- Storage
- 4 °C
- Storage Comment
- DDX60 antibody can be stored at 4 °C for three months. At -20 °C stable for up to one year.
- Expiry Date
- 12 months
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- Target
- DDX60 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 60 (DDX60))
- Alternative Name
- DDX60 (DDX60 Products)
- Synonyms
- 9830118M07 antibody, RGD1560014 antibody, DExD/H-box helicase 60 antibody, DEAD (Asp-Glu-Ala-Asp) box polypeptide 60 antibody, DEXD/H-box helicase 60 antibody, DDX60 antibody, Ddx60 antibody
- Background
- DDX60 is a DEXD/H Box RNA helicase and the human homolog of the S. cerevisiae Ski2, a cofactor of RNA exosome. Expression of DDX60 increases after viral infection and binds to endogenous RIG-I, a cytoplasmic viral RNA sensor, as well as other RIG-I-like receptors such as MDA5 and LGP2. These proteins activate TBK1 and IKK-e, signaling IRF3 and NF-kB-responsive genes in the innate immune response pathway. DDX60 is required for the RIG-I- or MDA5-dependent response to viral infection, but is dispensable for TLR3-mediated signaling.
- Molecular Weight
- 188 kDa
- Gene ID
- 55601
- NCBI Accession
- NP_060101
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