Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
Purification
conc. tissue culture supernatant
Clone
31C4
Isotype
IgG1
Application Notes
For western blots of yeast protein samples (cell and nuclear lysates), try MCA-31C4 diluted 1/10,000, followed by chemiluminescent detection (ECL). For non-ECL western detection methods, try MCA-31C4 diluted 1/1,000 to 1/5,000. For immunofluorescence on yeast cells, try MCA-31C4 diluted 1/500 to 1/5,000.
Restrictions
For Research Use only
Format
Liquid
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store at 4°C short term or -20°C long term.
Target
NSR1
(Nsr1p (NSR1))
Alternative Name
Nsr1p
Synonyms
SHE5 antibody, Nsr1p antibody, NSR1 antibody
Background
Nsr1p is localized in the nucleolus and binds to telomeric single-stranded oligonucleotide repeat sequence of the type and binds to the single-stranded telomeric repeat sequence (TG1-3)n. It has a role in ribosomal RNA maturation and possibly in transport of proteins into the nucleus.