mCherry antibody

Catalog No. ABIN1580414

Product Details anti-mCherry Antibody

Target: mCherry (mCherry Fluorescent Protein)

Reactivity: Discosoma

Host: Mouse

Clonality: Monoclonal

Conjugate: This mCherry antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Purification: affinity purified antibody

Clone: 1C51

Isotype: IgG2a

Application Details

Application Notes: Try at dilutions of 1:500 and higher for immunofluorescence. For western blots try at 1:2,000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4°C short term or -20°C long term.

Target Details for mCherry

Target: mCherry (mCherry Fluorescent Protein)

Alternative Name: Cherry Dsred (mCherry Products)

Background: MCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in transfection and transgenic experiments. The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a approx. 27 kDa protein isolated originally from the jellyfish Aequoria victoria. GFP was the basis of the 2008 Nobel Prize in Chemistry, awarded to Osamu Shimomura, Martin Chalfie and Roger Tsien, specifically “for the discovery and development of the green fluorescent protein, GFP“. GFP was shown to fluoresce on contact with molecular oxygen, requiring no other cofactors, and so can be expressed in fluorescent form in essentially any prokaryotic or eukaryotic cell.
The mCherry protein is derived from DsRed, a red fluorescent protein from so-called disc corals of the genus Discosoma. DsRed is similar in size and properties to GFP, but, obviously, produces a red rather than a green fluorochrome. The original DsRed was engineered extensively in the Tsien lab to prevent it from forming tetramers and dimers and to modify and improve the spectral properties. Several further cycles of mutation, directed modification and evolutionary selection produced mCherry, which has an excitation maximum at 587 nm and and emission maximum at 610 nm.
We expressed the mCherry protein sequence shown in some publications in bacteria, purified out the mCherry and raised a rabbit polyclonal antibody. This was affinity purified and was found to stain a band of the expected size in HEK293 cells transfected with a vector designed to express mCherry which was obtained from Clontech.