ALDOC antibody (C-Term)

Catalog No. ABIN1580409

Product Details anti-ALDOC Antibody

Target: ALDOC (Aldolase C, Fructose-Bisphosphate (ALDOC))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Cow, Pig, Mammalian

Host: Mouse

Clonality: Monoclonal

Conjugate: This ALDOC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Purification: affinity purified antibody

Clone: 1A1

Isotype: IgG1

Application Details

Application Notes: The antibody solution can be used at dilutions 1:1,000-2,000 for immunofluorescence. For western blots try at 1:2,000-5,000. A suitable control tissue is brain cell lysates.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4°C short term or -20°C long term.

Target Details for ALDOC

Target: ALDOC (Aldolase C, Fructose-Bisphosphate (ALDOC))

Alternative Name: Aldolase C (ALDOC Products)

Synonyms: ALDC antibody, MGC69434 antibody, ALDOC antibody, aldoc antibody, AI847350 antibody, AU040929 antibody, Aldo3 antibody, Scrg2 antibody, ALDCAA antibody, F16dip7 antibody, RATALDCAA antibody, aldocl antibody, wu:fj56h04 antibody, zgc:112357 antibody, QccE-21970 antibody, aldolase, fructose-bisphosphate C antibody, aldolase, fructose-bisphosphate C L homeolog antibody, aldolase C, fructose-bisphosphate, b antibody, aldolase C, fructose-bisphosphate antibody, aldolase A, fructose-bisphosphate antibody, aldolase C, fructose-bisphosphate, a antibody, Fructose-bisphosphate aldolase C antibody, ALDOC antibody, aldoc.L antibody, aldoc antibody, aldocb antibody, Aldoc antibody, ALDOA antibody, aldoca antibody

Background: Aldolases are glycolytic enzymes that catalyze the reversible aldol cleavage of fructose 1,6-bisphosphate and fructose-1-phosphate to dihydroxyacetone phosphate and either glyceraldehyde 3-phosphate or glyceraldehyde, respectively. Three aldolase isozymes are found in mammals, specifically aldolases A, B, and C, each of which is encoded by a separate gene.Aldolase A is generally considered to be a muscle enzyme. Northern analysis of cultured cells suggests that it is present in both neurons and glia. Aldolase B is considered to be a liver-specific enzyme and it is transcriptionally activated by signals from hormones and dietary factors. In the adult, aldolase C is the brain-specific isozyme, with low but detectable activity in fetal tissues. Aldolase C shares 81% amino acid identity with aldolase A and 70% identity with aldolase B. Earlier studies using isozyme-specific antibodies report its location in gray matter astrocytes and cells of the pia mater. In situ hybridization of mouse central nervous system using isozyme-specific probes revealed that aldolase A and C are expressed in complementary cell types: aldolase A mRNA is found in neurons, aldolase C message is detected in astrocytes, some cells of the pia mater, and Purkinje cells. Aldolase C can in some situations be used as an astrocyte marker. However Purkinje cells of the cerebellum contain high levels of the enzyme, so the enzyme is not totally astrocyte specific. MCA-1A1 was raised against C-terminal 23 amino acids of the human aldolase C protein, the sequence is KYEGSGEDGGAAAQSLYIANHAY. The HGNC name for this protein is ALDOC.