Avi-Tag antibody

Catalog No. ABIN1574261

Product Details anti-Avi-Tag Antibody

Target: Avi-Tag

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This Avi-Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Sequence: GLNDIFEAQKIEWHE

Specificity: Avi Tag Antibody, mAb, Mouse recognizes C-terminal Avi tagged fusion proteins and slightly recognizes N-terminal Avi tagged fusion proteins in Western Blot.

Cross-Reactivity (Details): Avi Tag Antibody, mAb, Mouse recognizes C-terminal Avi tagged fusion proteins and slightly recognizes N-terminal Avi tagged fusion proteins in Western Blot.

Purification: Protein A affinity column

Immunogen: Avi peptide conjugated - KLH

Clone: 1D11D10

Isotype: IgG2a

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.2-1 µg/mL FACS: 1-3 µg for 1 x 106 cells ICC/IF: 1-3 µg/mL ELISA: 0.02-0.05 µg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

References for anti-Avi-Tag antibody (ABIN1574261)

Nogal, McCoy, van Gils, Cottrell, Voss, Andrabi, Pauthner, Liang, Messmer, Nedellec, Shin, Turner, Ozorowski, Sanders, Burton, Ward: "HIV envelope trimer-elicited autologous neutralizing antibodies bind a region overlapping the N332 glycan supersite." in: Science advances, Vol. 6, Issue 23, pp. eaba0512, (2020) (PubMed).

Sheridan, Fong, DAlessandro, Bentley: "Widespread Backtracking by RNA Pol II Is a Major Effector of Gene Activation, 5' Pause Release, Termination, and Transcription Elongation Rate." in: Molecular cell, Vol. 73, Issue 1, pp. 107-118.e4, (2019) (PubMed).

Hill, Martinko, Nguyen, Wells: "Human antibody-based chemically induced dimerizers for cell therapeutic applications." in: Nature chemical biology, Vol. 14, Issue 2, pp. 112-117, (2019) (PubMed).

Iovino, Orihuela, Moorlag, Molema, Bijlsma: "Interactions between blood-borne Streptococcus pneumoniae and the blood-brain barrier preceding meningitis." in: PLoS ONE, Vol. 8, Issue 7, pp. e68408, (2014) (PubMed).

Target Details for Avi-Tag

Target: Avi-Tag

Alternative Name: Avi Tag (Avi-Tag Products)

Target Type: Tag

Background: Avi tag is a biotin-acceptor peptide, GLNDIFEAQKIEWHE. The 15-residue peptide served as a substrate mimic for biotin ligase (BirA), which usually recognizes the much larger protein domain. Avi Tag Antibody is a useful tool in analysis of Avi fusion proteins.