NA
Reactivity: Influenza A Virus H5N1
WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. Western Blot: 1-2 µg/mL
Restrictions
For Research Use only
Format
Lyophilized
Buffer
PBS, pH 7.4, containing 0.02 % sodium azide
Preservative
Sodium azide
Precaution of Use
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Storage
4 °C/-20 °C
Storage Comment
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Influenza A (H1N1) virus is a subtype of influenzavirus A and the most common cause of influenza (flu) in humans. A strain of swine-origin H1N1 was responsible for the 2009 flu pandemic. Neuraminidase (NA), a single-pass type II membrane homotetramer, is a major membrane glycoproteins found on the surface of influenza virus. It cleaves the hemagglutinin HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. Therefore, NA is described as a receptor-destroying enzyme which facilitates virus release and efficient spread of the progeny virus from cell to cell. H1N1 NA Antibody (3F11D7), mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from Mouse immunized with purified recombinant full-length Influenza A (H1N1) 2009 virus NA protein (GenBank: CY040001).