GFP antibody

Catalog No. ABIN1574242

Product Details anti-GFP Antibody

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Goat

Clonality: Polyclonal

Conjugate: This GFP antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Specificity: GFP Antibody, pAb, Goat specifically reacts with fusion proteins containing GFP epitope tag. This antibody recognizes all variants of GFP proteins such as eGFP, AcGFP1 and cGFP. It slightly recognizes a variety of variants RFP proteins such as tdTomato, mOrange, DsRed, DsRed2 and mCherry.

Cross-Reactivity (Details): GFP Antibody, pAb, Goat specifically reacts with fusion proteins containing GFP epitope tag. This antibody recognizes all variants of GFP proteins such as eGFP, AcGFP1 and cGFP. It slightly recognizes a variety of variants RFP proteins such as tdTomato, mOrange, DsRed, DsRed2 and mCherry.

Purification: Immunoaffinity chromatography

Immunogen: purified recombinant full-length GFP protein

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.2-1 µg/mL Immunoprecipitation: 1-5 µg/mL Other Applications: user optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP (GFP Products)

Synonyms: green fluorescent protein antibody, gfp antibody

Background: Green fluorescence protein (GFP) is a 27 kDa protein derived from jellyfish Aequorea victoria. It emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP has become a very useful tool as a fusion protein to report gene expression, to trace cell lineage and to define subcellular protein localizations. YFP differs from GFP due to a mutation at T203Y. GFP Antibody, pAb, Goat is developed in goat using purified recombinant full-length GFP protein.