Influenza A H1N1 PB1 antibody (C-Term)

Catalog No. ABIN1574132

Product Details anti-Influenza A H1N1 PB1 Antibody

Target: Influenza A H1N1 PB1 (PB1) (Influenza Polymerase Basic 1 (PB1))

Binding Specificity: C-Term

Reactivity: Influenza A Virus H1N1

Host: Rabbit

Clonality: Polyclonal

Application: Western Blotting (WB)

Specificity: H1N1 PB1 Antibody detects influenza A (H1N1) PB1 protein.

Purification: Immunoaffinity chromatography

Immunogen: KLH-coupled synthetic peptide from C-terminal of Influenza A (H1N1) 2009 virus PB1 protein (GenBank: CY040005).

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/mL.Other Applications: user optimized.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

References for anti-Influenza A H1N1 PB1 antibody (ABIN1574132)

Liedmann, Hrincius, Guy, Anhlan, Dierkes, Carter, Wu, Staeheli, Green, Wolff, McCullers, Ludwig, Ehrhardt: "Viral suppressors of the RIG-I-mediated interferon response are pre-packaged in influenza virions." in: Nature communications, Vol. 5, pp. 5645, (2014) (PubMed).

Target Details for Influenza A H1N1 PB1

Target: Influenza A H1N1 PB1 (PB1) (Influenza Polymerase Basic 1 (PB1))

Alternative Name: H1N1 PB1

Synonyms: polymerase PB1 antibody, PB1 antibody

Target Type: Influenza Protein

Background: Influenza A (H1N1) virus is a subtype of influenzavirus A and it is the most common cause of influenza (flu) in humans. A strain of swine-origin H1N1 was responsible for the 2009 flu pandemic. Influenza RNA-dependent RNA polymerase is composed of three subunits PB1, PA, and PB2. PB1 is the core of the complex and accounts for the polymerase activity. H1N1 PB1 Antibody is developed in rabbit using a KLH-coupled synthetic peptide from C-terminal of Influenza A (H1N1) 2009 virus PB1 protein (GenBank: CY040005).