ENO2/NSE antibody (C-Term)

Catalog No. ABIN1574104

Product Details anti-ENO2/NSE Antibody

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ENO2/NSE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Specificity: Rabbit Anti-NSE Polyclonal Antibody detects endogenous levels of human, mouse, and rat NSE protein. It may cross-reacts with alpha and beta enolase according to sequence homology.

Cross-Reactivity (Details): Rabbit Anti-NSE Polyclonal Antibody detects endogenous levels of human, mouse, and rat NSE protein. It may cross-reacts with alpha and beta enolase according to sequence homology.

Purification: Immunoaffinity chromatography

Immunogen: KLH-coupled synthetic peptide from C-terminal of human NSE

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.5-1 µg/mLImmunoprecipitation (IP): 2-10 µg/mg of lysateImmunohistochemistry: 5-10 µg/mLOther Applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Target Details for ENO2/NSE

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Alternative Name: NSE (ENO2 Products)

Synonyms: ENO2 antibody, DKFZp459B1817 antibody, NSE antibody, AI837106 antibody, D6Ertd375e antibody, Eno-2 antibody, RNEN3 antibody, eno3 antibody, wu:fc09h05 antibody, zgc:92418 antibody, enolase 2 antibody, enolase 2 (gamma, neuronal) antibody, enolase 2, gamma neuronal antibody, enolase 2, gamma, neuronal antibody, ENO2 antibody, Eno2 antibody, eno2 antibody

Background: Enolase is glycolytic enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. Mammalian enolase has three subunits (alpha, beta, and gamma) that can form homo and heterodimers. Homodimers of gamma enolase are neuronal-specific. NSE (neuron specific enolase) is expressed primarily in neurons. It is also found in elevated levels in plasma in certain neoplasias including pediatric neuroblastoma and small cell lung cancer. NSE is widely used as a diagnostic marker in a variety of clinical assays.Rabbit Anti-NSE Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from C-terminal of human NSE (Swiss Prot: P09104).