WDR77 antibody (C-Term)

Catalog No. ABIN1574092

Product Details anti-WDR77 Antibody

Target: WDR77 (WD Repeat Domain 77 (WDR77))

Binding Specificity: C-Term

Reactivity: Human, Orang-Utan

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WDR77 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Specificity: Rabbit Anti-MEP50 Polyclonal Antibody detects endogenous levels of human MEP50 protein. It is predicted to react with sumatran orangutan MEP50 protein according to sequence homology.

Cross-Reactivity (Details): Rabbit Anti-MEP50 Polyclonal Antibody detects endogenous levels of human MEP50 protein. It is predicted to react with sumatran orangutan MEP50 protein according to sequence homology.

Purification: Immunoaffinity chromatography

Immunogen: KLH-coupled synthetic peptide from carboxyl terminal of human MEP50

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 1-2 µg/mLOther Applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Target Details for WDR77

Target: WDR77 (WD Repeat Domain 77 (WDR77))

Alternative Name: MEP50 (WDR77 Products)

Synonyms: MEP-50 antibody, MEP50 antibody, Nbla10071 antibody, RP11-552M11.3 antibody, p44 antibody, p44/Mep50 antibody, 2610003I18Rik antibody, 2610312E17Rik antibody, C79984 antibody, p44/MEP50 antibody, RGD1310479 antibody, mep50 antibody, valois antibody, WDR77 antibody, zgc:65780 antibody, zgc:77274 antibody, WD repeat domain 77 antibody, WD repeat domain 77 S homeolog antibody, WDR77 antibody, Wdr77 antibody, wdr77.S antibody, wdr77 antibody

Background: MEP50 (methylosome protein 50), also known as WDR77, is a component of protein arginine methyltransferase complex containing at least PRMT5, CLNS1A and MEP50. It binds histone H2A selectively among core histones, and mediates transcriptional repression of protein arginine methyltransferase PRMT5. MEP50 is a WD repeat protein which interacts with an arginine protein JBP1. MEP50 plays a critical role in methylosome activity and may regulate the transfer of Sm proteins to the SMN (survival of motor neurons) complex, an early step in the assembly of U snRNPs. In addition, MEP50 may function to mediate the interaction of multiple substrates in the methylosome.Rabbit Anti-MEP50 Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from carboxyl terminal of human MEP50. (Swiss Prot: Q9BQA1).

Pathways: Ribonucleoprotein Complex Subunit Organization