GFAP antibody (AA 250-300)

Catalog No. ABIN1574085

Product Details anti-GFAP Antibody

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Binding Specificity: AA 250-300

Reactivity: Human, Rat, Orang-Utan

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFAP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Specificity: Rabbit Anti-GFAP Polyclonal Antibody detects endogenous levels of human and rat GFAP. It is predicted to react with sumatran orangutan GFAP protein according to sequence homology.

Cross-Reactivity (Details): Rabbit Anti-GFAP Polyclonal Antibody detects endogenous levels of human and rat GFAP. It is predicted to react with sumatran orangutan GFAP protein according to sequence homology.

Purification: Immunoaffinity chromatography

Immunogen: KLH-coupled peptide from within AA 250-300 of human GFAP.

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.5-1 µg/mLImmunohistochemistry: 5-10 µg/mLOther Applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

References for anti-GFAP antibody (ABIN1574085)

Long, Watts, Li, Shen, Muir, Huang, Boggs, Suri, Duong: "The effects of perturbed cerebral blood flow and cerebrovascular reactivity on structural MRI and behavioral readouts in mild traumatic brain injury." in: Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, Vol. 35, Issue 11, pp. 1852-61, (2015) (PubMed).

Talley Watts, Shen, Deng, Chemello, Duong: "Manganese-Enhanced Magnetic Resonance Imaging of Traumatic Brain Injury." in: Journal of neurotrauma, Vol. 32, Issue 13, pp. 1001-10, (2015) (PubMed).

Target Details for GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Alternative Name: GFAP (GFAP Products)

Synonyms: GFAP antibody, AI836096 antibody, cb345 antibody, etID36982.3 antibody, gfapl antibody, wu:fb34h11 antibody, wu:fk42c12 antibody, xx:af506734 antibody, zgc:110485 antibody, glial fibrillary acidic protein antibody, GFAP antibody, LOC100136168 antibody, gfap antibody, Gfap antibody

Background: GFAP (Glial fibrillary acidic protein) is a member of intermediate filament (IF) protein family which consists mainly of cytokeratin, GFAP, desmin, and vimentin. Like other IF proteins, GFAP is composed of an amino terminal head domain, a central rod domain, and a carboxyl terminal tail domain. The amino terminal region of GFAP shares considerable sequence divergence with other IF proteins, and probably participates in mediation of the tissue specific functions. GFAP intermediate filament serves as a useful marker of astroglia in the brain since it is specifically expressed in astrocytes and certain other astroglia. This protein may be required for communications between Bergmann glia and Purkinje cells during long-term depression induction and maintenance.Rabbit Anti-GFAP Polyclonal Antibody is developed in rabbit using a synthetic KLH-coupled peptide from within 250-300 of human GFAP (Swiss Prot: P14136).