Geminin antibody (AA 50-100)

Catalog No. ABIN1574066

Product Details anti-Geminin Antibody

Target: Geminin (GMNN) (Geminin, DNA Replication Inhibitor (GMNN))

Binding Specificity: AA 50-100

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Geminin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Specificity: Geminin Antibody, pAb, Rabbit detects endogenous levels of human geminin protein.

Purification: Immunoaffinity chromatography

Immunogen: KLH-coupled synthetic peptide within AA 50-100 of human geminin protein .

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.5-1 µg/mLFlow cytometry: 1-3 µg for 1 x 106 cellsOther applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

References for anti-Geminin antibody (ABIN1574066)

Wang, Kirschner: "Emi1 preferentially inhibits ubiquitin chain elongation by the anaphase-promoting complex." in: Nature cell biology, Vol. 15, Issue 7, pp. 797-806, (2013) (PubMed).

Target Details for Geminin

Target: Geminin (GMNN) (Geminin, DNA Replication Inhibitor (GMNN))

Alternative Name: Geminin (GMNN Products)

Synonyms: CG3183 antibody, Dm Geminin antibody, Dm geminin antibody, Dmel\\CG3183 antibody, Gem antibody, Geminin antibody, anon-WO0257455.1 antibody, gem antibody, l(2)k14019 antibody, v(2)k09107 antibody, sb:cb254 antibody, zgc:66217 antibody, GB16871 antibody, GMNN antibody, geminin antibody, dyak_GLEANR_833 antibody, DyakGE24654 antibody, GE24654 antibody, LOC100049517 antibody, AW546347 antibody, geminin, DNA replication inhibitor antibody, CG3183 gene product from transcript CG3183-RA antibody, geminin antibody, GE24654 gene product from transcript GE24654-RA antibody, GMNN antibody, geminin antibody, gmnn antibody, LOC410093 antibody, Dyak\geminin antibody, Gmnn antibody

Background: Geminin inhibits DNA replication by preventing the incorporation of the MCM complex into the prereplication complex (pre-RC). It is absent during the G1 phase, accumulates during the S, G2, and M phases, and disappears at the time of the metaphase-anaphase transition. Geminin contains 212 amino acids and has a destruction box sequence of RRTLKVIQP. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle.Geminin Antibody, pAb, Rabbit is developed in rabbit hosts using a KLH-coupled synthetic peptide within residues 50-100 of human geminin protein (Swiss Prot: O75496).

Pathways: EGFR Signaling Pathway, DNA Replication