AP2M1 antibody (N-Term)

Catalog No. ABIN1574063

Product Details anti-AP2M1 Antibody

Target: AP2M1 (Adaptor-Related Protein Complex 2, mu 1 Subunit (AP2M1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AP2M1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Rabbit Anti-AP2M1 Polyclonal Antibody detects endogenous levels of human AP2M1 protein. Sequence homology predicts that it will also react with mouse and rat AP2M1 proteins.

Cross-Reactivity (Details): Rabbit Anti-AP2M1 Polyclonal Antibody detects endogenous levels of human AP2M1 protein. Sequence homology predicts that it will also react with mouse and rat AP2M1 proteins.

Purification: Immunoaffinity chromatography

Immunogen: Synthetic peptide (KLH-coupled) from N-terminal of human AP2M1 protein

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL Western blot: 0.5-1 µg/mL
Other applications: user optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Target Details for AP2M1

Target: AP2M1 (Adaptor-Related Protein Complex 2, mu 1 Subunit (AP2M1))

Alternative Name: AP2M1 (AP2M1 Products)

Synonyms: AP50 antibody, CLAPM1 antibody, mu2 antibody, Ap50 antibody, Clapm1 antibody, cb34 antibody, Ap2m1 antibody, dpy-23 antibody, sb:cb34 antibody, zgc:55711 antibody, zgc:85653 antibody, wu:fa97a10 antibody, ap2m1-a antibody, ap2m1 antibody, zgc:56643 antibody, AP2M1 antibody, adaptor related protein complex 2 mu 1 subunit antibody, adaptor-related protein complex 2, mu 1 subunit antibody, adaptor-related protein complex 2, mu 1 subunit, a antibody, adaptor related protein complex 2 mu 1 subunit L homeolog antibody, adaptor-related protein complex 2, mu 1 subunit, b antibody, AP-2 complex subunit mu-1 antibody, clathrin coat assembly protein AP50 antibody, clathrin coat assembly protein ap50 antibody, AP2M1 antibody, Ap2m1 antibody, ap2m1a antibody, ap2m1.L antibody, ap2m1b antibody, ap2m1 antibody, cgd8_950 antibody, PY06523 antibody, PVX_118455 antibody, PVX_123590 antibody, CpipJ_CPIJ003697 antibody

Background: AP2M1 is a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunit family. AP2 is a heterotetramer composed of two large adaptins (either alpha-type subunit AP2A1 or AP2A2 paired with beta-type subunit AP2B1), a medium adaptin (mu-type subunit AP2M1) and a small adaptin (sigma-type subunit AP2S1). These component link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration.Rabbit Anti-AP2M1 Polyclonal Antibody is developed in rabbit hosts using a synthetic peptide (KLH-coupled) from N-terminal of human AP2M1 protein (Swiss Prot: Q96CW1).

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, EGFR Downregulation, SARS-CoV-2 Protein Interactome