PIN1
Reactivity: Human, Mouse, Rat, Green Monkey
WB, IHC (p)
Host: Mouse
Monoclonal
855CT1-7-5
unconjugated
Application Notes
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/mL Western blot: 0.5-1 µg/mL Other applications: user-optimized
Restrictions
For Research Use only
Format
Lyophilized
Buffer
PBS, pH 7.4, containing 0.02 % sodium azide
Preservative
Sodium azide
Precaution of Use
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Storage
4 °C/-20 °C
Storage Comment
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
PIN1 is an essential peptidyl-prolyl isomerase that regulates mitosis, presumably by interacting with NIMA and attenuating its mitosis-promoting activity. It displays a preference for an acidic residue N-terminal to the isomerized proline bond. It catalyzes pSer/Thr-Pro cis/trans isomerizations. PIN1 has been implicated in tau pathologies that underlie Alzheimer's disease. PIN1 binds to tau-phosphorylated sites, specifically on the Thr231-Pro site, where it induces conformational changes. PIN1 may be pivotal in maintaining normal neuronal function and preventing age-dependent neurodegeneration.Rabbit Anti-PIN1 Polyclonal Antibody is developed in rabbit hosts using a KLH-coupled synthetic peptide within residues 50-100 of human PIN1 protein (Swiss Prot: Q13526).