Rabbit Anti-Akt (Ser473) Polyclonal Antibody detects endogenous human and mouse total Aktalpha, Aktbeta and Aktgamma proteins. It has not yet been tested with other species.
Cross-Reactivity (Details)
Reactivity to other species is not tested yet.
Purification
Immunoaffinity chromatography
Immunogen
Synthesized phosphopeptide (KLH conjugated) derived from human Akt around the phosphorylation site of pSer473 (Q-F-S473-Y-S)
AKT1
Reactivity: Human
WB, IHC, IF, IP
Host: Rabbit
Monoclonal
unconjugated
Application Notes
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/mL Western blot: 0.5-2.0 µg/mL Other applications: user-optimized
Restrictions
For Research Use only
Format
Lyophilized
Buffer
PBS, pH 7.4, containing 0.02 % sodium azide
Preservative
Sodium azide
Precaution of Use
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Storage
4 °C/-20 °C
Storage Comment
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Wu, Storey: "FoxO3a-mediated activation of stress responsive genes during early torpor in a mammalian hibernator." in: Molecular and cellular biochemistry, Vol. 390, Issue 1-2, pp. 185-95, (2014) (PubMed).
Li, Zhou, Zhang, Su, Hang, Zhao, Su, Zhou: "BIM induction of apoptosis triggered by EGFR-sensitive and resistance cell lines of non-small-cell lung cancer." in: Medical oncology (Northwood, London, England), Vol. 28, Issue 2, pp. 572-7, (2011) (PubMed).
Akt, also known as protein kinase B (PKB), a serine/ threonine kinase, is a critical enzyme in several signal transduction pathways involved in cell proliferation, apoptosis, angiogenesis, and diabetes. Akt is activated following its phosphorylation at two regulatory residues. Phosphorylation of threonine on the kinase domain, catalyzed by PDK1, is essential for Akt activation. Akt activity is augmented approximately 10-fold by phosphorylation at the serine on the hydrophobic motif by PDK2. Phosphorylation of Thr308 and Ser473 activates Akt alpha. Phosphorylation at Thr309 and Ser474 on Akt beta1 and beta2, and on Thr305 on Akt gamma result in their activation. Akt promotes cell survival by inhibiting apoptosis by phosphorylating and inactivating several targets, including Bad, forkhead transcription factors, c-Raf and caspase-9. The activation of Akt is negatively regulated by PTEN, a PIP3 specific phosphatase, and SHIP, a SH2-domain containing inositol 5-phosphatase.Rabbit Anti-Akt (Ser473) Polyclonal Antibody is developed in rabbit using a synthetic peptide corresponding to residues surrounding S473 of human, rat and mouse Akt alpha, also known as protein kinase B alpha (PKBalpha), Akt1, and RACalpha. In addition, this peptide is identical to the sequence surrounding S474 of human, rat and mouse Akt beta (Akt2) and S472 of human and mouse Akt gamma (Akt3).