Protein A antibody (HRP)

Catalog No. ABIN1573943

Product Details anti-Protein A Antibody (HRP)

Target: Protein A

Reactivity: Staphylococcus aureus

Host: Chicken

Clonality: Polyclonal

Conjugate: This Protein A antibody is conjugated to HRP

Application: ELISA, Western Blotting (WB)

Specificity: Chicken Anti-Protein A [HRP] Polyclonal Antibody is specific to protein A.

Immunogen: Purified recombinant protein A

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.05-1.0 µg/mL
Other applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, containing 1 % BSA and 0.01 % thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

References for anti-Protein A antibody (HRP) (ABIN1573943)

OHalloran, Wynne, Geoghegan: "Protein A is released into the Staphylococcus aureus culture supernatant with an unprocessed sorting signal." in: Infection and immunity, Vol. 83, Issue 4, pp. 1598-609, (2015) (PubMed).

Nguyen, Rocha, Chintalacharuvu, Beenhouwer: "Detection and quantification of Panton-Valentine leukocidin in Staphylococcus aureus cultures by ELISA and Western blotting: diethylpyrocarbonate inhibits binding of protein A to IgG." in: Journal of immunological methods, Vol. 356, Issue 1-2, pp. 1-5, (2010) (PubMed).

Kathirvel, Morgan, French, Morgan: "Overexpression of liver-specific cytochrome P4502E1 impairs hepatic insulin signaling in a transgenic mouse model of nonalcoholic fatty liver disease." in: European journal of gastroenterology & hepatology, Vol. 21, Issue 9, pp. 973-83, (2009) (PubMed).

Target Details for Protein A

Target: Protein A

Alternative Name: ProteinA (Protein A Products)

Synonyms: alpha protein antibody, repB antibody

Background: Chicken Anti-Protein A [HRP] Polyclonal Antibody is developed in chicken host animals using purified recombinant protein A. Chicken anti-Protein A [HRP] is highly purified from chicken yolks by immunoaffinity chromatography and then conjugated to horseradish peroxidase. Chicken anti-Protein A is suitable for ELISA and immunoblotting.