Strep Tag II antibody

Catalog No. ABIN1573896

Product Details

Target: Strep Tag II

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Sequence: NWSHPQFEK

Specificity: This Antibody recognizes either N-terminal or C-terminal tagged fusion proteins.

Purification: Immunoaffinity chromatography

Immunogen: This epitope tag peptide NWSHPQFEK conjugated - KLH

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mL
Other applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

References

Kahl, Settele, Knick, Haupts, Bosse-Doenecke: "Mabfilin and Fabfilin - New antibody-scaffold fusion formats for multispecific targeting concepts." in: Protein expression and purification, Vol. 149, pp. 51-65, (2018) (PubMed).

Zhang, Zhong, Lu, Zhang, Deng, Huang, Duanmu et al.: "Characterization of Ferredoxin-Dependent Biliverdin Reductase PCYA1 Reveals the Dual Function in Retrograde Bilin Biosynthesis and Interaction With Light-Dependent Protochlorophyllide Oxidoreductase ..." in: Frontiers in plant science, Vol. 9, pp. 676, (2018) (PubMed).

Kuwae, Momose, Nagamatsu, Suyama, Abe: "BteA Secreted from the Bordetella bronchiseptica Type III Secetion System Induces Necrosis through an Actin Cytoskeleton Signaling Pathway and Inhibits Phagocytosis by Macrophages." in: PLoS ONE, Vol. 11, Issue 2, pp. e0148387, (2016) (PubMed).

Sampath, Brazier, Avril, Bernabeu, Vigdorovich, Mascarenhas, Gomes, Sather, Esmon, Smith: "Plasmodium falciparum adhesion domains linked to severe malaria differ in blockade of endothelial protein C receptor." in: Cellular microbiology, Vol. 17, Issue 12, pp. 1868-82, (2015) (PubMed).

Miranda, Antelmann, Hepowit, Chavarria, Krause, Pritz, Bäsell, Becher, Humbard, Brocchieri, Maupin-Furlow: "Archaeal ubiquitin-like SAMP3 is isopeptide-linked to proteins via a UbaA-dependent mechanism." in: Molecular & cellular proteomics : MCP, Vol. 13, Issue 1, pp. 220-39, (2014) (PubMed).

Turner, Lavstsen, Berger, Wang, Petersen, Avril, Brazier, Freeth, Jespersen, Nielsen, Magistrado, Lusingu, Smith, Higgins, Theander: "Severe malaria is associated with parasite binding to endothelial protein C receptor." in: Nature, Vol. 498, Issue 7455, pp. 502-5, (2013) (PubMed).

Avril, Brazier, Melcher, Sampath, Smith: "DC8 and DC13 var genes associated with severe malaria bind avidly to diverse endothelial cells." in: PLoS pathogens, Vol. 9, Issue 6, pp. e1003430, (2013) (PubMed).

Welsh, Lu, He, Greenberg, Swartz: "Cell-free production of trimeric influenza hemagglutinin head domain proteins as vaccine antigens." in: Biotechnology and bioengineering, Vol. 109, Issue 12, pp. 2962-9, (2012) (PubMed).

Target Details

Target: Strep Tag II

Target Type: Tag

Background: Well-characterized antibodies for epitope tags consisting of short sequences have been widely used in the study of protein expression in various systems. The NWSHPQFEK, a nine-amino acid peptide that is capable of binding to the biotin pocket of streptavidin was engineered. Rabbit Anti-NWSHPQFEK Polyclonal Antibody is developed in rabbit using a synthetic NWSHPQFEK peptide conjugated to KLH.