VSV-g Tag antibody

Catalog No. ABIN1573887

Product Details anti-VSV-g Tag Antibody

Target: VSV-g Tag (VSV-G Epitope Tag)

Reactivity: Vesicular Stomatitis Virus (VSV)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VSV-g Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Sequence: YTDIEMNRLG K

Specificity: This Antibody recognizes C-terminal, N-terminal, and internal VSV-G tagged fusion proteins.

Purification: Immunoaffinity chromatography

Immunogen: VSV-G epitope tag peptide YTDIEMNRLGK conjugated - KLH

Isotype: IgG1

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by severalfactors, including secondary antibody affinity, antigen concentration,sensitivity of detection method, temperature, and length of incubations,etc. The suitability of this antibody for applications other than thoselisted below has not been determined. The following concentration rangesare recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mLWestern Blot Using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Kit is recommendet. 10 µg of this antibody is mixed with 10 mL of WB solution for 8 cm x 8 cm membrane.Immunoprecipitation (IP): 2-10 µg/mg of lysateOther applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

References for anti-VSV-g Tag antibody (ABIN1573887)

Schmid, Sachs, tenOever: "Mitogen-activated protein kinase-mediated licensing of interferon regulatory factor 3/7 reinforces the cell response to virus." in: The Journal of biological chemistry, Vol. 289, Issue 1, pp. 299-311, (2014) (PubMed).

Zhang, He, Zhou, Pang, Liu, Zhao, Chen: "In vitro inhibition of vesicular stomatitis virus replication by purified porcine Mx1 protein fused to HIV-1 Tat protein transduction domain (PTD)." in: Antiviral research, Vol. 99, Issue 2, pp. 149-57, (2013) (PubMed).

Kita, Bertolesi, Hehr, Johnston, McFarlane: "Neuropilin-1 biases dendrite polarization in the retina." in: Development (Cambridge, England), Vol. 140, Issue 14, pp. 2933-41, (2013) (PubMed).

Zhao, Dai, Liang, Liang: "Surface display of rice stripe virus NSvc2 and analysis of its membrane fusion activity." in: Virologica Sinica, Vol. 27, Issue 2, pp. 100-8, (2012) (PubMed).

Padmashali, Andreadis: "Engineering fibrinogen-binding VSV-G envelope for spatially- and cell-controlled lentivirus delivery through fibrin hydrogels." in: Biomaterials, Vol. 32, Issue 12, pp. 3330-9, (2011) (PubMed).

Target Details for VSV-g Tag

Target: VSV-g Tag (VSV-G Epitope Tag)

Alternative Name: VSV-G-Tag (VSV-g Tag Products)

Target Type: Tag

Background: Rabbit Anti-VSV-G-tag Polyclonal Antibody is supplied as a 40 µg aliquot at a concentration of 1 mg/ml in PBS, pH 7.4, containing 0.02% Sodium azide. It is purified by immunoaffinity chromatography.