His Tag antibody

Catalog No. ABIN1573881

Product Details anti-His Tag Antibody

Target: His Tag

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This His Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Sequence: HHHHHH

Specificity: Rabbit Anti-His-tag Polyclonal Antibody specifically reacts with fusion proteins containing either amino- or carboxy-terminal His epitope tags.

Purification: Immunoaffinity chromatography

Immunogen: His epitope tag peptide HHHHHH conjugated - KLH

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mLWestern blot using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Kit is recommendet. 10 µg of this antibody is mixed with 10 mL of WB solution for 8 cm x 8 cm membrane.Other applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

References for anti-His Tag antibody (ABIN1573881)

Zhang, Qiu, Li, Liu, Shi, Wang: "Preparation of a monoclonal antibody against the carcinoembryonic antigen, glypican‑3." in: Molecular medicine reports, Vol. 19, Issue 5, pp. 3889-3895, (2019) (PubMed).

Dan, Kang, Duan, Wang: "A cell-free system toward deciphering the post-translational modification barcodes of Oct4 in different cellular contexts." in: Biochemical and biophysical research communications, Vol. 456, Issue 3, pp. 714-20, (2015) (PubMed).

Zhao, Zhou, Yao, Wan, Cao, Zhang, Zhao, Li, Zhou, Li, Wei, Zhang, French, Dekker, Yang, Fisher, Tucker, Guo: "Foxp1/2/4 regulate endochondral ossification as a suppresser complex." in: Developmental biology, Vol. 398, Issue 2, pp. 242-54, (2015) (PubMed).

Chen, Yang, Pang, Peng, Dong, Liu, Ouyang, Ren: "Expression, purification and antibody preparation using different constructs of PCV2 capsid protein." in: International journal of biological macromolecules, Vol. 67, pp. 289-94, (2014) (PubMed).

Baldwin, Yamodo, Ranjan, Li, Mines, Marinkovic, Hanada, Oh, Chishti: "Human erythrocyte band 3 functions as a receptor for the sialic acid-independent invasion of Plasmodium falciparum. Role of the RhopH3-MSP1 complex." in: Biochimica et biophysica acta, Vol. 1843, Issue 12, pp. 2855-70, (2014) (PubMed).

Steiner, Lori, Boehm, Jenal: "Allosteric activation of exopolysaccharide synthesis through cyclic di-GMP-stimulated protein-protein interaction." in: The EMBO journal, Vol. 32, Issue 3, pp. 354-68, (2013) (PubMed).

Wood, Iverson-Cabral, Patton, Cummings, Cosgrove Sweeney, Totten: "Persistence, immune response, and antigenic variation of Mycoplasma genitalium in an experimentally infected pig-tailed macaque (Macaca nemestrina)." in: Infection and immunity, Vol. 81, Issue 8, pp. 2938-51, (2013) (PubMed).

Raimunda, Subramanian, Stemmler, Argüello: "A tetrahedral coordination of Zinc during transmembrane transport by P-type Zn(2+)-ATPases." in: Biochimica et biophysica acta, Vol. 1818, Issue 5, pp. 1374-7, (2012) (PubMed).

Tosca, Glass, Bronchain, Philippe, Ciapa: "PLC?, G-protein of the G?q type and cADPr pathway are associated to trigger the fertilization Ca2+ signal in the sea urchin egg." in: Cell calcium, Vol. 52, Issue 5, pp. 388-96, (2012) (PubMed).

Sundlisaeter, Edelmann, Hol, Sponheim, Küchler, Weiss, Udalova, Midwood, Kasprzycka, Haraldsen: "The alarmin IL-33 is a notch target in quiescent endothelial cells." in: The American journal of pathology, Vol. 181, Issue 3, pp. 1099-111, (2012) (PubMed).

Rosadini, Gawronski, Raimunda, Argüello, Akerley: "A novel zinc binding system, ZevAB, is critical for survival of nontypeable Haemophilus influenzae in a murine lung infection model." in: Infection and immunity, Vol. 79, Issue 8, pp. 3366-76, (2011) (PubMed).

Sandee, Miller: "High-yield expression of a catalytically active membrane-bound protein: human P450 oxidoreductase." in: Endocrinology, Vol. 152, Issue 7, pp. 2904-8, (2011) (PubMed).

Yang, Hatton-Ellis, Simpson: "The kinase Sgg modulates temporal development of macrochaetes in Drosophila by phosphorylation of Scute and Pannier." in: Development (Cambridge, England), Vol. 139, Issue 2, pp. 325-34, (2011) (PubMed).

Kaufer, Smejkal, Osterrieder: "The varicella-zoster virus ORFS/L (ORF0) gene is required for efficient viral replication and contains an element involved in DNA cleavage." in: Journal of virology, Vol. 84, Issue 22, pp. 11661-9, (2010) (PubMed).

Lee, Park, Takahashi, Wang: "The association of AMPK with ULK1 regulates autophagy." in: PLoS ONE, Vol. 5, Issue 11, pp. e15394, (2010) (PubMed).

Medici, Shore, Lounev, Kaplan, Kalluri, Olsen: "Conversion of vascular endothelial cells into multipotent stem-like cells." in: Nature medicine, Vol. 16, Issue 12, pp. 1400-6, (2010) (PubMed).

Target Details for His Tag

Target: His Tag

Alternative Name: His-tag (His Tag Products)

Target Type: Tag

Background: Rabbit Anti-His-tag Polyclonal Antibody recognizes the His tag placed at N-terminal, C-terminal, and internal regions of targeted proteins in transfected or transformed cells. Short-sequence epitope tags are common in the study of protein expression in several different expression systems. In particular, the His tag on the expressed recombinant proteins allows for efficient coupling to Ni2+ affinity resins and purification by single step chromatography.