DYKDDDDK Tag antibody

Catalog No. ABIN1573877

Product Details anti-DYKDDDDK Tag Antibody

Target: DYKDDDDK Tag

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DYKDDDDK Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Sequence: DYKDDDDK

Specificity: This Antibody recognizes C-terminal, N-terminal, and internal tagged fusion proteins.

Purification: Immunoaffinity chromatography

Immunogen: A synthetic peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated - KLH

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.01-0.05 µg/mL
Western blot: 0.1-1.0 µg/mLWestern Blot Using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Kit is recommendet. 10 µg of this antibody is mixed with 10 mL of WB solution for 8 cm x 8 cm membrane.Immunofluorescence:5-20 µg/mLFlow Cytometry:1-3 µg for 1 x 106 cellsOther applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

References for anti-DYKDDDDK Tag antibody (ABIN1573877)

Wang, Liu, Ye, Liu, Wu, Huang, Shi: "Ddx56 maintains proliferation of mouse embryonic stem cells via ribosome assembly and interaction with the Oct4/Sox2 complex." in: Stem cell research & therapy, Vol. 11, Issue 1, pp. 314, (2020) (PubMed).

Hoshii, Cifani, Feng, Huang, Koche, Chen, Delaney, Lowe, Kentsis, Armstrong: "A Non-catalytic Function of SETD1A Regulates Cyclin K and the DNA Damage Response." in: Cell, Vol. 172, Issue 5, pp. 1007-1021.e17, (2019) (PubMed).

Li, Liu, Feng, Wang, Das, Xu, Zhou, Sun, Guan, Xiong, Lei: "Glyceraldehyde-3-phosphate dehydrogenase is activated by lysine 254 acetylation in response to glucose signal." in: The Journal of biological chemistry, Vol. 289, Issue 6, pp. 3775-85, (2014) (PubMed).

Huang, Lv, Liu, Zha, Zhang, Jiang, Xiong, Lei, Guan: "The N-terminal phosphodegron targets TAZ/WWTR1 protein for SCF?-TrCP-dependent degradation in response to phosphatidylinositol 3-kinase inhibition." in: The Journal of biological chemistry, Vol. 287, Issue 31, pp. 26245-53, (2012) (PubMed).

Liu, Lv, Li, Xu, Zhou, Zhao, Xiong, Lei, Guan: "PP1 cooperates with ASPP2 to dephosphorylate and activate TAZ." in: The Journal of biological chemistry, Vol. 286, Issue 7, pp. 5558-66, (2011) (PubMed).

Zhou, Li, Feng, Hsiang, Xiong, Guan, Lei: "Targeted polyubiquitylation of RASSF1C by the Mule and SCF?-TrCP ligases in response to DNA damage." in: The Biochemical journal, Vol. 441, Issue 1, pp. 227-36, (2011) (PubMed).

Bedoya, Martínez, Rubio, Daròs: "Simultaneous equimolar expression of multiple proteins in plants from a disarmed potyvirus vector." in: Journal of biotechnology, Vol. 150, Issue 2, pp. 268-75, (2010) (PubMed).

Liu, Zha, Zhou, Zhang, Huang, Zhao, Li, Chan, Lim, Hong, Zhao, Xiong, Lei, Guan: "The hippo tumor pathway promotes TAZ degradation by phosphorylating a phosphodegron and recruiting the SCF{beta}-TrCP E3 ligase." in: The Journal of biological chemistry, Vol. 285, Issue 48, pp. 37159-69, (2010) (PubMed).

Zhang, Liu, Zha, Zhao, Yao, Zhao, Xiong, Lei, Guan: "TEAD transcription factors mediate the function of TAZ in cell growth and epithelial-mesenchymal transition." in: The Journal of biological chemistry, Vol. 284, Issue 20, pp. 13355-62, (2009) (PubMed).

Target Details for DYKDDDDK Tag

Target: DYKDDDDK Tag

Alternative Name: DYKDDDDK-Tag (DYKDDDDK Tag Products)

Target Type: Tag

Background: Rabbit Anti-DYKDDDDK-tag Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 µg aliquot at a concentration of 1 mg/ml in PBS, pH 7.4, containing 0.02% Sodium azide.