This Fc epsilon RI/FCER1A antibody is un-conjugated
Application
Flow Cytometry (FACS), Western Blotting (WB), ELISA, Functional Studies (Func)
Characteristics
The antibody recognizes an epitope within the a2 domain of the human high affinity IgE receptor (FceRIa). It inhibits binding of IgE to this receptor and is suitable to detect receptors expressed on primary cells and cell lines (e.g mast cells and basophils). It can also be used as weak to moderate cross-linking agent for IgE-independent triggering of effector cells and to detect FceRIa in ELISA and W-blots.
Purification
Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on anti-mouse IgG1 agarose