CCR5 antibody (AA 1-22)

Catalog No. ABIN1558080

Product Details anti-CCR5 Antibody

Target: CCR5 (Chemokine (C-C Motif) Receptor 5 (CCR5))

Binding Specificity: AA 1-22

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CCR5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Specificity: The mouse monoclonal antibody T21/8 recognizes an extracellular epitope on the N-teminus of CD195, an approximately 45 kDa G-protein coupled receptor 1 family protein expressed on resting T cells, monocytes, macrophages, and immature dendritic cells.

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: CCR5 peptide (Met1-Lys22) KLH conjugate

Clone: T21-8

Isotype: IgG1 kappa

Application Details

Application Notes: Western blotting: Do not boil cell lysates prior to SDS-PAGE.
Flow cytometry: Recommended dilution: 1-4 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CCR5 antibody (ABIN1558080)

Monde, Maeda, Tanaka, Harada, Yusa: "Gp120 V3-dependent impairment of R5 HIV-1 infectivity due to virion-incorporated CCR5." in: The Journal of biological chemistry, Vol. 282, Issue 51, pp. 36923-32, (2007) (PubMed).

Hüttenrauch, Pollok-Kopp, Oppermann: "G protein-coupled receptor kinases promote phosphorylation and beta-arrestin-mediated internalization of CCR5 homo- and hetero-oligomers." in: The Journal of biological chemistry, Vol. 280, Issue 45, pp. 37503-15, (2005) (PubMed).

Pollok-Kopp, Schwarze, Baradari, Oppermann: "Analysis of ligand-stimulated CC chemokine receptor 5 (CCR5) phosphorylation in intact cells using phosphosite-specific antibodies." in: The Journal of biological chemistry, Vol. 278, Issue 4, pp. 2190-8, (2003) (PubMed).

Target Details for CCR5

Target: CCR5 (Chemokine (C-C Motif) Receptor 5 (CCR5))

Alternative Name: CD195 / CCR5 (CCR5 Products)

Synonyms: CC-CKR-5 antibody, CCCKR5 antibody, CCR-5 antibody, CD195 antibody, CKR-5 antibody, CKR5 antibody, CMKBR5 antibody, IDDM22 antibody, Ckr5 antibody, Cmkbr5 antibody, CCR1-L antibody, GPCR antibody, CCR5 antibody, AM4-7 antibody, C-C motif chemokine receptor 5 (gene/pseudogene) antibody, chemokine (C-C motif) receptor 5 antibody, C-C motif chemokine receptor 5 antibody, CCR5 antibody, Ccr5 antibody

Background: C-C motif chemokine receptor 5,CD195 / CCR5 (also known as CKR-5) is a receptor for inflammatory CC-chemokines (characterized by a pair of adjacent cysteine residues), such as MIP-1 alpha, MIP-1 beta, or RANTES. It is a G protein-associated seven-pass transmembrane protein expressed on resting T cells with memory/effector phenotype, monocytes, macrophages and immature dendritic cells. This chemokine receptor regulates the activation and directed migration of leukocytes. Importantly, along with CD4, CD195 / CCR5 functions as a major receptor for HIV. Their ligand is the viral glycoprotein gp120.,CKR5, CCR5, CCCKR5, CMKBR5, IDDM22, CC-CKR-5

Gene ID: 1234

UniProt: P51681

Pathways: Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Regulation of Cell Size