MFAP5 antibody (C-Term)

Catalog No. ABIN155193

Product Details anti-MFAP5 Antibody

Target: MFAP5 (Microfibrillar Associated Protein 5 (MFAP5))

Binding Specificity: C-Term

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MFAP5 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This affinity-purified antibody is directed against Human MAGP-2 protein. A BLAST analysis was used to suggest cross reactivity with MAGP-2 protein from Human and chimpanzee based on 100 % homology with the immunizing sequence. Expect partial reactivity with MAGP-2 from Mouse, Dog and Rat sources based on partial (~75 %) sequence homology. Reactivity against homologues from other sources is not known.

Cross-Reactivity (Details): Species reactivity (tested):Human, Chimpanzee.

Purification: Immunoaffinity (from monospecific antiserum).

Immunogen: Synthetic peptide corresponding to a region near the carboxy terminal end of human MAGP-2 protein. Remarks: Full length protein sequence for human MAGP-2 (173 aa, predicted MW 19.6 kDa): 1 msllgpkvll flaafiitsd wiplgvnsqr gddvtqatpe tftedpnlvn dpatdetvla 61 vladiapstd dlaslseknt taecwdekft ctrlysvhrp vkqcihqlcf tslrrmyivn 121 keicsrlvck eheamkdelc rqmaglpprr lrrsnyfrlp pcenvdlqrp ngl

Application Details

Application Notes: This affinity-purified antibody has been tested for use in ELISA (1/5,000-1/20,000) andWestern blot (1/250-1/1,000). Specific conditions for reactivity should be optimized by theend user. Expect a band approximately 20-30 kDa in size corresponding to MAGP-2 proteinby Western blotting in the appropriate cell lysate or extract.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.95 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. Restore with deionized water r equivalent, this antibody is stable for one month at 2-8 °C as an undiluted liquid. For extended storage aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature.

Target Details for MFAP5

Target: MFAP5 (Microfibrillar Associated Protein 5 (MFAP5))

Alternative Name: MFAP5 (MFAP5 Products)

Background: MAGP-2 is a secreted, extracellular matrix protein which is a component of the elastin-associated microfibrils. It forms intermolecular disulfide bonds either with other MAGP-2 Molecules or with other components of the microfibrils. MAGP-2 is upregulated in some ovarian cancers and is associated with poor survival of ovarian cancer patients.Synonyms: MAGP2, MFAP-5, MP25, Microfibril-associated glycoprotein 2, Microfibrillar-associated protein 5

Gene ID: 8076

NCBI Accession: NP_003471

UniProt: Q13361