VEGFA
Reactivity: Human
WB, ELISA, Neut
Host: Goat
Polyclonal
unconjugated
Application Notes
ELISA: 1-15 μg/mL. Western blot: 2-10 μg/mL. Neutralization: Blocks the VEGF-A induced proliferation of ACE cell at a 1: 6 Molar ratio ofligand to antibody. Inhibits the binding of VEGF-A to the VEGF receptors 1 (Flt-1) and 2 (KDR). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
Buffer
H2O, Trehalose, Na-Phosphate, Polysorbart20
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
The lyophilized antibody, thought stable at RT, is best stored desiccated below 0 °C. Reconstituted anti-VEGF-A is stable at 2-8 °C for one month or (in aliquots) at - 20 °C for longer.
Target
VEGFA
(Vascular Endothelial Growth Factor A (VEGFA))
Human Vascular Endothelial Growth Factor VEGF165, a 23 kDa protein consisting of 165 amino acid residues, is produced as a homodimer. VEGF is a polypeptide growth factor and a member of the platelet-derived growth factor family. It is a specific mitogen for vascular endothelial cells and a strong angiogenic factor in vivo. Two high-affinity tyrosine kinase receptors for VEGF165 have been identified, VEGFR-1 (FLT-1), and VEGFR-2 (KDR). In addition to its action as a mitogen it is a potent vascular permeability factor (VPF) in vivo. VEGF165 is also a chemo attractant molecule for monocytes and endothelial cells. 5 different proteins are generated by differential splicing: VEGF121, VEGF145, VEGF165, VEGF189 and VEGF206. The most abundant form is VEGF165. Whereas VEGF121 and VEGF165 are secreted proteins, VEGF145, VEGF189 and VEGF206 are strongly cell-associated. The isoforms VEGF145, VEGF165 and VEGF189 bind to heparin with high affinity. VEGF165 is apparently a homo-dimer, but preparations of VEGF165 show some heterogeneity on SDS gels, depending on the secretion of different glycosylation patterns. All dimeric forms have similar biological activities but their bioavailability is very different. There is good evidence that different cells and tissues express different VEGF isoforms. The other members of this increasing growth factor family are VEGF-B, -C, -D and -E. Another member is the Placenta growth factor PlGF.Synonyms: VEGF, VEGFA, VPF, Vascular endothelial growth factor A, Vascular permeability factor