Prostate Specific Antigen antibody

Catalog No. ABIN1543584

Product Details anti-Prostate Specific Antigen Antibody

Target: Prostate Specific Antigen (PSA)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Prostate Specific Antigen antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Dot Blot (DB)

Purification: Protein A affinity column

Immunogen: Native PSA Antigen

Clone: A5D5-1

Isotype: IgG1

Application Details

Comment:

Other Applications have not been tested.

Restrictions: For Research Use only

Independent Validation (FACS)

Validation #029617 (Flow Cytometry)

by: Flow Cytometry & Cell Separation Facility, Purdue University

No.: #029617

Date: 02/22/2014

Lot Number: FAPS-A5D5-0003

Method validated: Flow Cytometry

Positive Control: LNCaP cells

Negative Control: - DU145 cells - Jurkat cells

Notes: Strong signal above isotype signal was observed in the positive control sample, while staining of similar level to isotype control was observed in both negative control samples.

Validation Images

Figure 1: LNCaP cells stained with anti-PSA (blue) or with isotype control (red).

Figure 2: DU145 cells stained with anti-PSA (blue) or with isotype control (red).

Figure 3: Jurkat cells stained with anti-PSA (blue) or with isotype control (red).

Full Methods

Primary Antibody:

• Antibody: Prostate Specific Antigen (PSA)
• Catalog number: ABIN1543584
• Lot number: FAPS-A5D5-0003

Secondary Antibody:

• Antibody: F(ab’)2 goat anti-mouse IgG-Alexa 647

Full Protocol:

• Positive and negative control cells were washed once with phosphate-buffered saline (PBS) and harvested with 0.05% trypsin / 0.025% EDTA.
• Detached cells were washed and resuspended in 0.5 mL 1X PBS containing 0.5% BSA.
• An equivalent amount of pre-warmed 4% paraformaldehyde was added and the cells were incubated for 10 min at 37°C.
• Cells were washed in 1X PBS containing 0.5% BSA and resuspended in 1 mL of ice-cold 90% methanol for 30 min on ice.
• Cells were spun out of the methanol and washed twice by resuspending in 1X PBS containing 0.5% BSA and 0.1% Tween 20
• Cells were resuspended in 100 µL 1X PBS containing 0.5% BSA.
• 1 µg of anti-PSA or mouse IgG1 isotype control were added to the cells and incubated for 30 min at room temperature
• Cells were washed twice with PBS/BSA/Tween 20
• Cells were resuspended in 100 µL 1X PBS containing 0.5% BSA
• Secondary antibody was added at a final dilution of 1:500 and incubated for 30 min at room temperature
• Cells were washed twice with PBS/BSA/Tween 20
• Cells were resuspended in 0.3 mL PBS/BSA
• Cells were analyzed on a FACSAria III (BD Biosciences) using a red laser (640 nm excitation / 660 nm emission).

Experimental Notes: None

Handling

Format: Liquid

Concentration: 1.8 mg/mL

Buffer: PBS, pH 7.4, 0.09% sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for Prostate Specific Antigen

Target: Prostate Specific Antigen (PSA)

Alternative Name: PSA (PSA Products)

Synonyms: APS antibody, KLK2A1 antibody, PSA antibody, hK3 antibody, KLK3 antibody, Klk1c10l2 antibody, RSGK-50 antibody, RSKG-50 antibody, rGK-3 antibody, Kal-3 antibody, Kal3 antibody, Klk3 antibody, AAP-S antibody, MP100 antibody, Psa antibody, R74825 antibody, goku antibody, kallikrein related peptidase 3 antibody, kallikrein 3 antibody, kallikrein 1-related peptidase C3 antibody, kallikrein B, plasma 1 antibody, aminopeptidase puromycin sensitive antibody, KLK3 antibody, Klk1c3 antibody, Klkb1 antibody, Npepps antibody

Pathways: Complement System