GAPDH antibody

Catalog No. ABIN1539789

Product Details anti-GAPDH Antibody

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: GAPDH recombinant protein is used to produce this monoclonal antibody.

Clone: 1A10A10

Isotype: IgG1

Application Details

Application Notes: IF: 1:25. WB: 1:1000. WB: 1:8000. WB: 1:2000~10000. IHC-P: 1:25. IHC-P: 1:25

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: GAPDH Antibody can be refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-GAPDH antibody (ABIN1539789)

Bai, Zhang, Hu, Liu, Wang, Li, Lin, Zhang, Sun, Du, Song: "Silencing DNA methyltransferase 1 (DNMT1) inhibits proliferation, metastasis and invasion in ESCC by suppressing methylation of RASSF1A and DAPK." in: Oncotarget, Vol. 7, Issue 28, pp. 44129-44141, (2018) (PubMed).

Zhang, Shi, Guo, Miao, Li: "Use of rhenium-188 for in vivo imaging and treatment of human cervical cancer cells transfected with lentivirus expressing sodium iodide symporter." in: Oncology reports, Vol. 36, Issue 4, pp. 2289-97, (2017) (PubMed).

Zhang, Chen, Wang, Gao, Jin: "The Role of Annexin A4 in Triple-Negative Breast Cancer Progression and Its Clinical Application." in: Annals of clinical and laboratory science, Vol. 46, Issue 5, pp. 515-21, (2017) (PubMed).

He, Pu, Yuan, Jia, Wang: "Nutrition deficiency promotes apoptosis of cartilage endplate stem cells in a caspase-independent manner partially through upregulating BNIP3." in: Acta biochimica et biophysica Sinica, Vol. 49, Issue 1, pp. 25-32, (2017) (PubMed).

Wang, Zhang, Huang, Xu, Zhou, Yu, Luo, Wen, Jia, Zheng: "Expression and prognostic significance of MYL9 in esophageal squamous cell carcinoma." in: PLoS ONE, Vol. 12, Issue 4, pp. e0175280, (2017) (PubMed).

Zhao, Li, Hao, Chen, Wu, Song, Zhang, Qiao, Li: "Peptide SS-31 upregulates frataxin expression and improves the quality of mitochondria: implications in the treatment of Friedreich ataxia." in: Scientific reports, Vol. 7, Issue 1, pp. 9840, (2017) (PubMed).

Wang, Liu, Tang, Deng, Chen, Zhu, Shen: "Hepatocellular Carcinoma Cells Induce Regulatory T Cells and Lead to Poor Prognosis via Production of Transforming Growth Factor-β1." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 38, Issue 1, pp. 306-18, (2016) (PubMed).

Boutry, El-Kadi, Suryawan, Steinhoff-Wagner, Stoll, Orellana, Nguyen, Kimball, Fiorotto, Davis: "Pulsatile delivery of a leucine supplement during long-term continuous enteral feeding enhances lean growth in term neonatal pigs." in: American journal of physiology. Endocrinology and metabolism, Vol. 310, Issue 8, pp. E699-E713, (2016) (PubMed).

Zhang, Wang, Guo, Liang, Jiang, Zhang, Li: "Molecular In Vivo Imaging Using a Noninvasive Cardiac-Specific MLC-2v Promoter Driven Dual-Gene Recombinant Lentivirus Monitoring System." in: PLoS ONE, Vol. 10, Issue 7, pp. e0133952, (2015) (PubMed).

Han, Xu, Xu, Chen, Zeng, Zhang, Cao, Kong, Tang, Mao: "SC06, a novel small molecule compound, displays preclinical activity against multiple myeloma by disrupting the mTOR signaling pathway." in: Scientific reports, Vol. 5, pp. 12809, (2015) (PubMed).

Wu, Liu, Wang, Xu, Meng, Gu: "Upregulation of microRNA-96 and its oncogenic functions by targeting CDKN1A in bladder cancer." in: Cancer cell international, Vol. 15, pp. 107, (2015) (PubMed).

Ma, Ou-Yang, Luo, Wang, Hou, Han, Liu, Schatten, Sun: "Cyclin O regulates germinal vesicle breakdown in mouse oocytes." in: Biology of reproduction, Vol. 88, Issue 5, pp. 110, (2013) (PubMed).

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH (GAPDH Products)

Synonyms: G3PD antibody, GAPD antibody, Gapd antibody, BEST:GH12586 antibody, CG12055 antibody, Dmel\\CG12055 antibody, GA3PDH antibody, GADPH antibody, GAP antibody, GAPDH antibody, GAPDH I antibody, GAPDH-1 antibody, GAPDH1 antibody, GAPDHI antibody, Gapdh antibody, Gapdh-1 antibody, Gapdh43E antibody, gadph antibody, gapdh antibody, gapdh-1 antibody, gh12586 antibody, cb609 antibody, gapd antibody, mg:bb02e05 antibody, wu:fb33a10 antibody, wu:ft80f05 antibody, KNC-NDS6 antibody, g3pd antibody, G3PDH antibody, glyceraldehyde-3-phosphate dehydrogenase antibody, Glyceraldehyde 3 phosphate dehydrogenase 1 antibody, glyceraldehyde-3-phosphate dehydrogenase S homeolog antibody, glyceraldehyde-3-phosphate dehydrogenase, type I antibody, olfactory receptor 8K3 antibody, GAPDH antibody, Gapdh antibody, Gapdh1 antibody, gapdh antibody, gapdh.S antibody, gapDH antibody, LOC100404960 antibody, LOC614985 antibody

Background: The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome.

Molecular Weight: 36053

Gene ID: 2597

NCBI Accession: NP_001243728, NP_002037

UniProt: P04406