This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen
This POLR1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1437-1465 amino acids from the C-terminal region of human POLR1A.
194kDa antibody, 3010014K16Rik antibody, RPA194 antibody, Rpo1-4 antibody, mRPA1 antibody, Rpa1 antibody, A190 antibody, RPA1 antibody, RPO1-4 antibody, RPO14 antibody, polymerase (RNA) I polypeptide A antibody, RNA polymerase I subunit A antibody, Polr1a antibody, POLR1A antibody
Background
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from RPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity).