Caspase 8 antibody (Cleaved-Asp384)

Catalog No. ABIN486727

Product Details anti-Caspase 8 Antibody

Target: Caspase 8 (CASP8)

Binding Specificity: AA 335-384, Cleaved-Asp384

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity:

Caspase 8 (Cleaved-Asp384) Antibody detects endogenous levels of fragment of activated Caspase 8 resulting from cleavage adjacent to Asp384.

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Caspase 8.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 ELISA: 1:40000

Comment:

Unigene-Number: Hs.599762 (NCBI Gene Symbol: CASP8)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

References for anti-Caspase 8 antibody (ABIN486727)

Liu, Huang, Liu, Li, Yang, Dou, Liu: "Calcium channel blocker verapamil accelerates gambogic acid-induced cytotoxicity via enhancing proteasome inhibition and ROS generation." in: Toxicology in vitro : an international journal published in association with BIBRA, Vol. 28, Issue 3, pp. 419-25, (2014) (PubMed).

Target Details for Caspase 8

Target: Caspase 8 (CASP8)

Alternative Name: Caspase 8 (CASP8 Products)

Background:

Synonyms: Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4, Caspase-8 precursor, FADD-homologous ICE/CED-3- like protease, FADD-like ICE, FLICE, ICE-like apoptotic protease 5, ICE8, MACH, MCH5, MORT1-associated CED-3 homolog

NCBI Gene Symbol: CASP8

Molecular Weight: 47 and 55 kDa

Gene ID: 841

OMIM: 211980

UniProt: Q14790

Pathways: Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades