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Vimentin antibody (AA 411-460)

Name: Vimentin antibody (AA 411-460)
SKU: ABIN1533459
Price: 341 USD
Availability: InStock

VIM Reactivity: Human, Mouse, Rat WB, IHC, ELISA Host: Rabbit Polyclonal unconjugated

Western blot analysis of extracts from HepG2 cells, treated with Adriamycin 0. (Vimentin antibody (AA 411-460))

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Vimentin Antibody. (Vimentin antibody (AA 411-460))

IHC

2 images

(1 reference)

Catalog No. ABIN1533459

Datasheet as PDF

Target See all Vimentin (VIM) Antibodies

Vimentin (VIM)
Binding Specificity
32

16

16

15

11

9

9

7

4

3

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 411-460
Reactivity
304

119

116

60

60

49

42

37

29

21

20

13

13

8

8

6

6

3

3

2

1

1

1

1

1

1

1

1

1
Human, Mouse, Rat
Host
157

130

11

6

6

5

2
Rabbit
Compatible Secondaries
Clonality
171

145
Polyclonal
Conjugate
197

15

13

12

9

7

3

3

3

3

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1
This Vimentin antibody is un-conjugated
Application
262

115

103

97

87

64

48

40

39

33

23

20

8

5

4

4

3

2

2

2

1

1

1

1

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Specificity

Vimentin Antibody detects endogenous levels of total Vimentin protein.

Purification

The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.

Purity

> 95 %

Immunogen

The antiserum was produced against synthesized peptide derived from human Vimentin.

Isotype

IgG

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VIM Reactivity: Human, Mouse WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB41034 unconjugated

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VIM Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human WB, IHC, ICC, IP Host: Mouse Monoclonal C8 unconjugated

anti-Vimentin (VIM) (AA 430-457), (C-Term) antibody
VIM Reactivity: Human WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB16691 unconjugated

anti-Vimentin (VIM) (AA 2-466) antibody
VIM Reactivity: Human WB, IHC, ELISA, FACS Host: Mouse Monoclonal 3A1F2 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Pig, Cow, Chicken, Dog, Cat, Goat WB, IHC, FACS, StM Host: Mouse Monoclonal VM452 unconjugated

Application Notes

WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

Comment

Unigene-Number: Hs.455493, Hs.628678 (NCBI Gene Symbol: VIM)

Restrictions

For Research Use only
Format

Liquid

Concentration

1 mg/mL

Buffer

phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Stable at -20°C for at least 1 year.

Expiry Date

12 months
Yu, Zeidel, Hill: "Cellular expression profile for interstitial cells of cajal in bladder - a cell often misidentified as myocyte or myofibroblast." in: PLoS ONE, Vol. 7, Issue 11, pp. e48897, (2012) (PubMed).
Target

Vimentin (VIM)

Alternative Name

Vimentin (VIM Products)

Background

Synonyms: VIME, vimentin
NCBI Gene Symbol: VIM

Molecular Weight

53 kDa

Gene ID

7431

OMIM

193060

UniProt

P08670

Pathways

Caspase Cascade in Apoptosis