Merlin antibody (pSer10)

Catalog No. ABIN1532019

Product Details anti-Merlin Antibody

Target: Merlin (NF2) (Neurofibromin 2 (NF2))

Binding Specificity: AA 1-50, pSer10

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Merlin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificity:

Merlin (Phospho-Ser10) Antibody detects endogenous levels of Merlin only when phosphorylated at Ser10.

PhosphorylationH:S10 M:S10 R:S6

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Merlin around the phosphorylation site of Ser10.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

Comment:

Unigene-Number: Hs.187898 (NCBI Gene Symbol: NF2)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

References for anti-Merlin antibody (ABIN1532019)

Yu, Zeidel, Hill: "Cellular expression profile for interstitial cells of cajal in bladder - a cell often misidentified as myocyte or myofibroblast." in: PLoS ONE, Vol. 7, Issue 11, pp. e48897, (2012) (PubMed).

Target Details for Merlin

Target: Merlin (NF2) (Neurofibromin 2 (NF2))

Alternative Name: Merlin (NF2 Products)

Synonyms: ACN antibody, BANF antibody, SCH antibody, NF2 antibody, merlin antibody, zgc:194620 antibody, zgc:194631 antibody, NF2a antibody, nf2 antibody, neurofibromin 2 antibody, neurofibromin 2a (merlin) antibody, neurofibromin 2b (merlin) antibody, neurofibromin 2 (merlin) S homeolog antibody, NF2 antibody, Nf2 antibody, nf2a antibody, nf2b antibody, nf2.S antibody

Background:

Synonyms: MERL, moesin-ezrin-radizin-like protein, neurofibromatosis type 2 gene, neurofibromatosis-2, Neurofibromin 2, NF-2, NF2, SCH, Schwannomerlin, schwannomerlin, Schwannomin

NCBI Gene Symbol: NF2

Molecular Weight: 69 kDa

Gene ID: 4771

OMIM: 101000

UniProt: P35240

Pathways: Cell-Cell Junction Organization