SAPK, JNK (AA 151-200), (pTyr185) antibody

Catalog No. ABIN1531885

Product Details

Target: SAPK, JNK

Binding Specificity: AA 151-200, pTyr185

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA

Specificity:

SAPK/JNK (Phospho-Tyr185) Antibody detects endogenous levels of SAPK/JNK only when phosphorylated at Tyr185.

PhosphorylationH:Y185/223 M:Y185/223 R:Y185/223

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human SAPK/JNK around the phosphorylation site of Tyr185.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

Comment:

Unigene-Number: Hs.138211, Hs.654461, Hs.125503 (NCBI Gene Symbol: MAPK9)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details

Target: SAPK, JNK

Background:

Synonyms: JNK-46, JNK1-alpha-2, MAPK8, MK08, Mitogen-activated protein kinase 8, PRKM8, SAPK gamma, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1, kinase JNK1, p54 gamma

NCBI Gene Symbol: MAPK9

Molecular Weight: 48 kDa

Gene ID: 5599, 5601, 5602

OMIM: 601158

UniProt: P45983, P45984, P53779