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Vimentin antibody (pSer83)

Name: Vimentin antibody (pSer83)
SKU: ABIN482371
Price: 225.5 USD
Availability: InStock

VIM Reactivity: Human, Mouse, Rat WB, IHC, ELISA Host: Rabbit Polyclonal unconjugated

Western blot analysis of extracts from 293 cells treated with paclitaxel 1uM 24h, using Vimentin (Phospho-Ser83) Antibody. (Vimentin antibody (pSer83))

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Vimentin (Phospho-Ser83) Antibody. (Vimentin antibody (pSer83))

IHC

2 images

Catalog No. ABIN482371

Datasheet as PDF

Target See all Vimentin (VIM) Antibodies

Vimentin (VIM)
Binding Specificity
32

16

16

15

11

9

9

7

4

3

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 56-105, pSer83
Reactivity
305

120

117

60

60

49

42

37

29

21

20

13

13

8

8

6

6

3

3

2

1

1

1

1

1

1

1

1

1
Human, Mouse, Rat
Host
158

130

11

6

6

5

2
Rabbit
Compatible Secondaries
Clonality
172

145
Polyclonal
Conjugate
198

15

13

12

9

7

3

3

3

3

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1
This Vimentin antibody is un-conjugated
Application
263

115

104

97

88

64

48

40

39

33

23

20

8

5

4

4

3

2

2

2

1

1

1

1

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Specificity

Vimentin (Phospho-Ser83) Antibody detects endogenous levels of Vimentin only when phosphorylated at Ser83.
PhosphorylationH:S83 M:S83 R:S83

Purification

The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity

> 95 %

Immunogen

The antiserum was produced against synthesized peptide derived from human Vimentin around the phosphorylation site of Ser83.

Isotype

IgG

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Application Notes

WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

Comment

Unigene-Number: Hs.642813 (NCBI Gene Symbol: VIM)

Restrictions

For Research Use only
Format

Liquid

Concentration

1 mg/mL

Buffer

phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Stable at -20°C for at least 1 year.

Expiry Date

12 months
Target

Vimentin (VIM)

Alternative Name

Vimentin (VIM Products)

Background

Synonyms: VIME, vimentin
NCBI Gene Symbol: VIM

Molecular Weight

53 kDa

Gene ID

7431

OMIM

193060

UniProt

P08670

Pathways

Caspase Cascade in Apoptosis