NCF1 antibody (pSer359)

Catalog No. ABIN1531723

Product Details anti-NCF1 Antibody

Target: NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

Binding Specificity: AA 331-380, pSer359

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Specificity:

p47 phox (Phospho-Ser359) Antibody detects endogenous levels of p47 phox only when phosphorylated at Ser359.

PhosphorylationH:S359

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human p47 phox around the phosphorylation site of Ser359.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000

Comment:

Unigene-Number: Hs.647047, Hs.655201 (NCBI Gene Symbol: NCF1)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

References for anti-NCF1 antibody (ABIN1531723)

Kim, Cho, Mun, Kim, Kim, Kim, Son, Jeon, Kim, Jeong, Jang, Kim, Yang: "Multi-Functional MPT Protein as a Therapeutic Agent against Mycobacterium tuberculosis." in: Biomedicines, Vol. 9, Issue 5, (2021) (PubMed).

Target Details for NCF1

Target: NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

Alternative Name: p47 Phox (NCF1 Products)

Synonyms: NCF1A antibody, NOXO2 antibody, SH3PXD1A antibody, p47phox antibody, Ncf-1 antibody, p47 antibody, neutrophil cytosolic factor 1 antibody, NCF1 antibody, Ncf1 antibody

Background:

Synonyms: 47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-1, NCF-47K, NCF1, Neutrophil cytosol factor 1, Neutrophil cytosolic factor 1, Neutrophil NADPH oxidase factor 1, P47 phox, p47-phox

NCBI Gene Symbol: NCF1

Molecular Weight: 44 kDa

Gene ID: 653361

OMIM: 233700

UniProt: P14598

Pathways: PI3K-Akt Signaling