PKC antibody (pThr497)

Catalog No. ABIN1531508

Product Details anti-PKC Antibody

Target: PKC (PKC pan) (Protein Kinase C (pan) (PKC pan))

Binding Specificity: AA 623-672, pThr497

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Specificity:

PKC-pan (Phospho-Thr497) Antibody detects endogenous levels of PKC-pan only when phosphorylated at Thr497.

PhosphorylationH:T497/500/507/566/514/512/538/410 M:T497/500/505/566/514/513/538/410 R:T497/500/505/566/514/513/538/410

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human PKC-pan around the phosphorylation site of Thr497.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000

Comment:

Unigene-Number: Hs.531704, Hs.708867, Hs.460355, Hs.155342, Hs.580351, Hs.631564, Hs.333907, Hs.498570, Hs.496255 (NCBI Gene Symbol: PRKCA)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for PKC

Target: PKC (PKC pan) (Protein Kinase C (pan) (PKC pan))

Alternative Name: PKC (PKC pan Products)

Synonyms: PKC antibody, PKCI antibody, Prkc antibody, Prkcc antibody, RATPKCI antibody, protein kinase C, gamma antibody, Prkcg antibody

Background:

Synonyms: KPCA, PKC III, PKC-A, PKC-III, PKC-alpha, PRKACA, PRKCA, Protein kinase C, alpha type, kinase PKC-alpha PKC-delta, PRKCD, kinase PKC-delta, nPKC-delta PKC-epsilon, PKCEA, PRKCE, epsilon type, kinase PKC-epsilon, nPKC-epsilon

NCBI Gene Symbol: PRKCA

Molecular Weight: 76 kDa

Gene ID: 5578, 5579, 5580

OMIM: 176960

UniProt: P17252, P05771, Q05655, Q02156, P05129, P24723, Q04759, Q05513

Pathways: Positive Regulation of Response to DNA Damage Stimulus