14-3-3 theta antibody (pSer232)

Catalog No. ABIN1531476

Product Details anti-14-3-3 theta Antibody

Target: 14-3-3 theta (YWHAQ)

Binding Specificity: AA 196-245, pSer232

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 theta antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Specificity:

14-3-3 thet/tau (Phospho-Ser232) Antibody detects endogenous levels of 14-3-3 thet/tau only when phosphorylated at Ser232.

PhosphorylationH:S232 M:S232

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human 14-3-3 thet/tau around the phosphorylation site of Ser232.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000

Comment:

Unigene-Number: Hs.170839 (NCBI Gene Symbol: YWHAQ)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for 14-3-3 theta

Target: 14-3-3 theta (YWHAQ)

Abstract: YWHAQ Products

Background:

Synonyms: 14-3-3 protein T-cell, 14-3-3 protein tau, 1433T, 143T, HS1 protein, YWHAQ

NCBI Gene Symbol: YWHAQ

Molecular Weight: 27 kDa

Gene ID: 5350

OMIM: 172405

UniProt: P27348

Pathways: Apoptosis, Myometrial Relaxation and Contraction