WNK1 antibody (pThr60)

Catalog No. ABIN1531249

Product Details anti-WNK1 Antibody

Target: WNK1 (WNK Lysine Deficient Protein Kinase 1 (WNK1))

Binding Specificity: AA 24-73, pThr60

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WNK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificity:

WNK1 (Phospho-Thr58) Antibody detects endogenous levels of WNK1 only when phosphorylated at Thr58.

PhosphorylationH:T60 M:T58 R:T58

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human WNK1 around the phosphorylation site of Thr58.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000

Comment:

Unigene-Number: Hs.356604, Hs.656067 (NCBI Gene Symbol: WNK1)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for WNK1

Target: WNK1 (WNK Lysine Deficient Protein Kinase 1 (WNK1))

Alternative Name: WNK1 (WNK1 Products)

Background:

Synonyms: WNK lysine deficient protein kinase 1, KDP, PRKWNK1, PROTEIN KINASE, LYSINE-DEFICIENT 1

NCBI Gene Symbol: WNK1

Molecular Weight: 250 kDa

Gene ID: 65125

OMIM: 145260

UniProt: Q9H4A3